急性低氧影响大鼠脑血流动力学的机制研究

High altitude cerebral edema (HACE) is the most dangerous altitude sickness and high mortality. HACE was considered as cytotoxicedema mainly in the past. But more and more evidence suggests suggested that HACE is probable angioedema. It will be directly related to select these measures of prevention and treatment of HACE that which type of edema is dominant. We compare the hemodynamics indicators of cerebral by different vasoactive substances blocker under different oxygen environment, and combine with the overall hemodynamic changes to investigate the mechanism of occurrence and development of HACE. To investigate the biochemistry, blood gas, the expression of cerebrovascular occludin, brain tissue morphology and ultrastructure, and redox level of brain under the different oxygen environments, to analyze the type of edema and cerebral hemodynamics mechanism. This study will exposit the initiating keys of HACE’s occurrence and development process by analyzing the changes of cerebral vascular resistance and cerebral oxygen consumption, and combined with the overall hemodynamic changes in different hypoxia on cerebral hemodynamics and cerebral metabolic function. Completion of the research will be providing an important theoretical basis for the prevention and treatment of HACE, and the research results of this basic theory has further clinical utility.

高原性脑水肿（HACE, high altitude cerebral edema）在高原病中最为凶险、病死率高。过去仅认为HACE主要是细胞中毒性脑水肿，但越来越多的证据表明它的形成过程中也伴有血管源性脑水肿，对于HACE到底是以哪种类型水肿占主导优势直接关系到HACE的有效预防和治疗措施的选择。本课题通过应用不同血管活性因子干预，实时、动态地对比观察不同低氧下脑的血流动力学指标，并对不同急性低氧环境下大鼠血液生化、血气、脑血管Occludin 蛋白表达、脑组织形态及超微结构和脑氧化还原水平的观察，结合全身血流动力学的改变，探索HACE在其发生、发展过程中不同急性低氧影响脑水肿的类型和脑血流动力学变化机制。经过观察低氧下脑血管阻力和脑耗氧量的改变，分析不同程度急性低氧对脑血流动力学和脑代谢功能的影响，揭示HACE发生发展的始动环节，这将为预防和治疗HACE提供更具有临床实用性的理论。