新疆野山杏总有机酸对动物脂质代谢影响及机理研究

The wild apricot in Xinjiang exhibits resisting drought and cold environment, protecting against wind, fixing sand, and helping check runoff. Wild apricots have been planted massively in Xinjiang, and the area has been more than 200,000 Mu. As such, to development wild apricots resource in Xinjiang will be of important significance to local economic growth. With the changes of modern lifestyles and eating habits, the fatty metabolic disorder diseases are increasing day by day. Based on our previous study on this project, we will seperate and purify organic acids from wild apricots in Xinjiang, and carry out the following researchs: ①To investigate the effects on improving fatty metabolism of organic acids from wild apricots in animals. ②Using RT-PCR, fluorescent quantitation PCR, and immunohistochemistry techniques to observe the effects of organic acids from wild apricots on gene and protein expression of receptor relevant to fatty acid metabolic in animal liver cells. To explore the influence of organic acid from wild apricots on the animal fat metabolic at the molecular levels. ③The effect of organic acids on macrophage in vivo and in vitro will be observed. To investigate the influence of organic acids from wild apricots on the animal fat metabolic at the cellular level. In this study, improving fatty metabolism effects and mechanism of the organic acids from wild apricot in Xinjiang will be investigated, resulting in providing references for further processing wild apricots, and development them for functional foods materials to improve lipid metabolic disorder.

新疆野山杏具有较强耐旱抗寒的适应能力和优良防风保土固沙性能，在全疆多个地区大面积种植，其面积已超过20万亩，故开发利用新疆野山杏资源对带动地方经济的发展具有十分重要的意义。随着人们生活方式和饮食习惯的改变，脂质代谢紊乱性疾病日渐增多。本项目以丰富的新疆野山杏为研究材料，从果肉中分离纯化总有机酸：①通过动物试验探讨总有机酸对动物脂质代谢的作用；②利用RT-PCR和荧光定量PCR以及免疫组化技术，探究总有机酸对动物肝脏细胞脂肪酸代谢相关受体的蛋白及基因表达的影响，解明总有机酸对动物脂质代谢分子水平的作用机理；③通过考察对动物体内泡沫细胞和ox-LDL体外诱导RAW264.7巨噬细胞源性泡沫细胞形成的影响，探明总有机酸对脂质代谢细胞水平的作用机理。通过上述研究查明新疆野山杏总有机酸对脂质代谢的改善功能和作用机理。为新疆野山杏的深加工和将其开发为非药物治疗脂质代谢紊乱的功能性食品原料提供科学依据。

本项目以丰富的新疆野山杏为研究材料，从果肉中分离纯化了总有机酸，并利用RP-HPLC法同时测定了果肉中10种有机酸成分；通过动物试验研究了野山杏果肉总有机酸对动物脂质代谢的影响，通过测定载脂蛋白的含量、制作肝脏组织切片、利用荧光实时定量PCR（Real-time fluorescent quantitative PCR，RT-PCR）技术测定总有机酸对动物肝脏细胞脂肪酸代谢相关受体基因表达的影响；研究了野山杏果肉总有机酸体外和体内的抗氧化活性。通过这些研究阐明了野山杏果肉总有机酸对脂质代谢的改善功能，并揭示了野山杏果肉总有机酸对脂质代谢作用机理，其作用机理是通过抗氧化、调节载脂蛋白的含量、上调肝脏PPAR-α和LDL-R的基因表达量以及改善肝功能等方面产生调节脂质代谢紊乱的作用。同时研究了野山杏果肉总有机酸中含量最高的成分柠檬酸对高脂血症诱导的糖代谢紊乱的改善作用及其作用机理，其中的柠檬酸成分能够调节高脂血症小鼠的血脂水平，并能够有效调节糖代谢过程，提高机体对胰岛素的敏感性，减弱高脂血症小鼠的胰岛素抵抗，增加葡萄糖在机体中的利用率。本研究结果为新疆野山杏的深加工和将其开发为非药物治疗代谢紊乱性疾病的功能性食品原料提供了科学依据。

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