低频超声激活树突状细胞诱导抗前列腺癌免疫效应的应用研究

Microbubbles mediated low-power and low-frequency ultrasound in the treatment of prostate cancer research has achieved good results , but the low -power and low-frequency ultrasound induced biological effects occur mainly in the mechanism of acoustic cavitation effects by microbubbles mediated. Microbubbles mediated low-power and low-frequency ultrasound can activate dendritic cell mediated immune response tumor or not is still unclear. Our previous study found that microbubbles mediated low-power and low-frequency ultrasound can inhibit prostate cancer that hinder factor VEGF expression in prostate cancer differentiation and maturation of dendritic cells to suppress tumor immunity , the subject of research by understanding microbubbles mediated low-power and low-frequency ultrasound in the inhibition of prostate cancer based on the expression of VEGF can further promote the differentiation and maturation of dendritic cells and thus initiate tumor immunity. The first, systematic study of different frequencies , power and low frequency ultrasonic irradiation time alone or in combination microbubbles influence on VEGF expression in prostate cancer ; then low-frequency ultrasound therapeutic effect on dendritic cell differentiation and maturation ; the third is maturation dendritic cell activation tumor immunity , immune effects. Aims to systematically evaluate microbubbles mediated activation of the low-frequency and low- power ultrasound in prostate cancer tumor immunity effect , provide a new theoretical foundation for the microbubbles mediated low-power and low-frequency ultrasound treatment of prostate cancer.

微泡介导的低频低功率超声在前列腺癌治疗的基础研究中已经取得了良好的结果，但目前低频低功率超声所致的生物学效应发生机制主要集中在微泡介导的声空化效应，低频低功率超声能否激活树突状细胞介导的肿瘤免疫效应目前仍不清楚。课题组前期研究发现，微泡介导的低频低功率超声能够抑制前列腺癌VEGF这一阻碍前列腺癌树突状细胞分化成熟从而抑制肿瘤免疫的因子表达，本课题通过以下研究深入了解微泡介导的低频低功率超声在抑制前列腺癌VEGF表达基础上能否进一步促进树突状细胞的分化成熟进而启动肿瘤免疫。1.系统性研究不同频率、功率及辐照时间的低频超声单独或联合微泡对前列腺癌VEGF表达的影响；2.治疗性低频超声在树突状细胞分化成熟上的作用；3.分化成熟的树突状细胞激活肿瘤免疫，产生免疫效应。旨在系统性评价微泡介导的低频低功率超声在激活前列腺癌肿瘤免疫中的作用，为微泡介导的低频低功率超声治疗前列腺癌提供新的理论基础。

前列腺癌是一个严重威胁老年男性的恶性肿瘤，临床治疗仍然存在难点。本研究计划从建立可控、可重复的低频超声联合微泡造影剂通过诱导抗肿瘤免疫来治疗前列腺癌的技术研发平台出发，首先在原有低频超声治疗仪基础上进一步改进了低频超声治疗仪，实现了一台主机变频使用；通过体内及体外实验证实可以通过抑制VEGF的表达，实现促进DC细胞的分化成熟，进而促进抗肿瘤免疫的发生；低频超声联合微泡能够抑制VEGF的表达，从而实现诱导抗肿瘤免疫效应，抑制肿瘤的生长。低频超声联合微泡促进了抗肿瘤免疫对前列腺癌的治疗效果，为进一步应用治疗提供了理论基础。.低频超声联合微泡治疗前列腺癌如果有效，将会对于前列腺癌的治疗具有极大的社会效益，一种新的无创的治疗方法作为现有治疗方法的一个有效补充，造福广大的患者。

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