基于蛋白组学和遗传学的可见光光催化杀菌机理研究

Disinfection is an essential step to remove pathogenic microbial contamination in water/wastewater treatment. However, some bacterium have evolved resistance mechanism toward traditional disinfection methods such as chlorination. More crucially, potential carcinogenic and mutagenic disinfection by-products (DBPs) have recently been identified during chlorination. Recently, photocatalysis has emerged as a promising alternative to control microbial contamination in environment. Yet, to date, the precise molecular mechanism(s) causes bacterial inactivation and what are the bacterial cellular responses during the photocatalytic disinfection are poorly understood. This study aims at investigating the visible-light-driven (VLD) photocatalytic inactivation mechanisms of bacteria at the molecular level. Proteomic and qPCR analysis will be used to identify the potential bacterial gene products involved in photocatalytic inactivation. Then the results will be verified by genetic study using isogenic single deletion mutants of respective identified bacterial genes (and proteins) involved in VLD photocatalytic inactivation. The effects of physiological factors (e.g. pH, temperature, nutrient profile and bacteria growth stage) on the proteome of bacteria will be link to inactivation to identify the critical parameters. The combination of proteomics and genetics approaches throughout this study will allow us to have a comprehensive picture of what are the cellular responses to VLD photocatalysis and the mechanisms of VLD photocatalytic inactivation at molecular level. More importantly, according to the results of this study, the research team can recommend the best operation condition(s) to obtain the most efficient VLD photocatalytic inactivation of bacteria in water and wastewater disinfection treatments.

消毒是水处理中去除致病性生物危害的一个必要步骤。但是传统的水消毒技术（例如 氯杀菌消毒）不能有效杀灭某些抗性细菌，并可能产生危害更加大的消毒副产物。最近，光催化技术日渐发展成为一种潜在的能够取代传统水处理技术的新方法。而目前光催化灭菌过程中分子尺度上的机制仍缺乏深入的认识。本项目拟研究可见光光催化技术杀灭细菌的机理，通过蛋白质组学， 荧光定量PCR和基因敲除技术，在分子尺度上探究并验证细菌的光催化灭活与抗性机制，并考察多种细菌生长生理因子（例如pH，温度，营养物底物浓度和细菌生长阶段）对细菌蛋白质表达及其相应的灭活性能的影响。在此基础上提出可见光光催化杀菌的最佳操作条件，同时达到高效去除水体中的细菌污染。该项目的开展将在蛋白和基因水平上深入认识可见光光催化灭活细菌的机制，丰富半导体可见光光催化杀菌的理论知识，为开发新型高效环境光催化水体杀菌技术提供新的思路。

消毒是水处理中去除致病性生物危害的一个必要步骤。但是传统的水消毒技术（例如 氯杀菌消毒）不能有效杀灭某些抗性细菌，并可能产生危害更加大的消毒副产物。最近，光催化技术日渐发展成为一种潜在的能够取代传统水处理技术的新方法。但是，光催化杀菌过程机理非常复杂，目前缺乏深入的认识，光催化剂开发与光催化系统设计缺乏必要的理论指导。本研究围绕光催化水处理技术，开发了高效稳定的光催化剂; 并且探索了光催化消毒的对水体微生物稳定性的影响; 利用蛋白质组学与基因组学探究了光催化杀菌过程中的微生物机制;在此基础上, 开发了一套中试规模的光催化杀菌反应器。共发表论文7篇，其中第一/通讯作者的SCI论文5篇，4篇发表在环境国际知名期刊（中科院SCI分区1区）。

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