DNA四面体的细胞摄取，转运及其机制研究

Self-assembled DNA nanostructures have emerged as a type of nano-biomaterials with precise structures, versatile functions and numerous applications, such as drug delivery. The study of the cellular uptake and transport process of DNA nanostructure are of great significance for its application in the field of biological and medicine. DNA tetrahedron is a great model of DNA nanostructure since it can be easily synthesis with different size and biologically modify. In this project, we want develop and use multiple cell image tools, such as super resolution microscopy, total internal reflection microscopy, live cell imaging technology to study the cell uptake, transport, localization and discharge process of DNA tetrahedron. We also want to explore the related mechanism of these biological processes. Base on the analysis of the relationship between the size, modification of DNA tetrahedron and their interaction of cell, we want to develop the reliable method to control the fate of DNA tetrahedron in the cell, which can be helpful to strengthen its biological function. This project could deepen the understanding of the cellular mechanisms of how the nucleic acids uptake by the cell. On the other hand, this project would also be very helpful to the biological applications of DNA nanostructures such as drug delivery and gene regulation.

DNA纳米技术是目前纳米领域一个新兴的研究热点，其细胞摄取、转运过程的研究对其在生物医药领域的应用具有重要意义。本项目拟以DNA四面体作为研究对象，使用包括超分辨细胞成像技术、全内反射显微技术、活细胞成像技术等成像手段，系统研究不同尺寸大小、偶联不同生物分子的DNA四面体的细胞摄取、转运、定位及外排的过程，并探索其相关机制。在此基础上，还拟通过修饰特定生物功能化片段改变DNA四面体的细胞摄取方式及细胞器定位，并强化其生物学功能。本项目的实施不但能够加深对核酸类物质进入细胞机制的理解，也能为利用DNA纳米结构进行药物运输、基因调控等生物学应用奠定基础。