表面等离子体共振增强的近红外荧光成像用于肾癌的早期诊断和靶向治疗研究

Near-infrared fluorescence imaging is a novel multi-disciplines crossed fields based on it's deep tissue pentration and has attracted hot attentions in cancer areas including early diagnosis, targeted therapy and the machanisim research. This project aims at developing surface plasmon resonce enhanced near-infrared II fluorescent probe (Carbon nanotube, Graphene, Ag2S) labled with anti-Tim-3 for the targeted near-infrared imaging of renal cancer cell and animals with high multi spatio-temporal resolution, high sensitivity and low background. With the help of total internal reflection fluorescence microscope (TIRFM), confocal laser scanning microscope (CLSM) and microfluidic chips, the mechanism of scape from immunity of Tim-3 in tumor microenvirnment and origin of renal cancer will be elucidated deeply. We will set up the novel molecular recognition methods and technologies at single molecule, single cell and animal level and finaly realize the theranostic' Imaging. The research results of this project has tremendous research values and potential applications in the bioanalysis of life science, single cell detection, in-vivo imaging, clinical diagnosis and therapy.

近红外荧光成像是分析化学、材料科学、影像学与生命科学相交叉的前沿课题，以其独特的组织穿透能力在肿瘤领域中展现出巨大的潜力，对于肿瘤的早期诊断、疗效观察、机理研究均有重要意义。本项目拟采用金属表面等离子共振增强的第二窗口近红外(NIR II)荧光材料（包括碳纳米管、石墨烯量子点、Ag2S）来标记T细胞免疫球蛋白粘蛋白Tim-3抗体，并通过抗Tim-3与T-淋巴细胞和肾癌组织之间的特异性相互作用实现肾癌细胞和动物活体的高时空分辨力、高灵敏度和高靶向性的近红外荧光分子成像。借助激光共聚焦显微镜、全内反射荧光显微镜、微流控芯片等技术深入研究Tim-3在肿瘤微环境中的免疫逃逸机制和肾癌的发病机理，同时构建单细胞和活体内分子识别的新技术和新方法，实现肾癌的早期诊断和治疗一体化。相关研究成果对于建立高分辨率的单分子成像和高灵敏度、高靶向性的癌症早期诊断和治疗的方法和技术具有理论指导意义和实际应用价值。