Paper-based fluorescence resonance energy transfer assay for directly detecting nucleic acids and proteins

Paper-based fluorescence resonance energy transfer assay (FRET) is gaining great interest in detecting macro-biological molecule. It is difficult to achieve conveniently and fast detection for macro-biological molecule. Herein, a graphene oxide (GO)-based paper chip (glass fiber) integrated with fluorescence labeled single-stranded DNA (ssDNA) for fast, inexpensive and direct detection of biological macromolecules (proteins and nucleic acids) has been developed. In this paper, we employed the Cy3/FAMlabeled ssDNA as the reporter and the GO as quencher and the original glass fiber paper as data acquisition substrates. The chip which was designed and fabricated by a cutting machine is a miniature biosensor that monitors fluorescence recovery from resonance energy transfer. The hybridization assays and fluorescence detection were all simplified, and the surface of the chip did not require immobilization or washing. A Nikon Eclipse was employed as excited resource and a commercial digital camera was employed for capturing digital images. This paper-based microfluidics chip has been applied in the detection of proteins and nucleic acids. The biosensing capability meets many potential requirements for disease diagnosis and biological analysis. (C) 2016 Published by Elsevier B.V.

纸基荧光共振能量转移分析（FRET）在检测大分子生物分子方面引起了极大的关注。对于大分子生物分子，很难实现便捷快速的检测。在此，我们开发了一种基于氧化石墨烯（GO）的纸芯片（玻璃纤维），它结合了荧光标记的单链DNA（ssDNA），可用于快速、廉价且直接地检测生物大分子（蛋白质和核酸）。在本文中，我们采用Cy3/FAM标记的ssDNA作为报告分子，GO作为猝灭剂，原始的玻璃纤维纸作为数据采集基底。通过切割机设计和制造的芯片是一种微型生物传感器，可监测共振能量转移产生的荧光恢复。杂交分析和荧光检测都得到了简化，并且芯片表面不需要固定或清洗。采用尼康Eclipse作为激发源，使用商用数码相机拍摄数字图像。这种纸基微流控芯片已应用于蛋白质和核酸的检测。其生物传感能力满足疾病诊断和生物分析的许多潜在需求。（C）2016年由爱思唯尔B.V.出版