蓝藻NDH-1-PSI在胁迫条件下的形成机制及其生理功能分析

Cyanobacterial NDH-1 can interact with PSI to form an NDH-1-PSI supercomplex via a light-harvesting antenna CpcG2-phycobilisome (PBS) and the supercomplex is exclusively involved in cyclic electron transport around PSI (NDH-CET). However, the forming mechanism and physiological function of NDH-1-PSI under stress conditions remains elusive. Recently, we found that quality of light including green light and red light altered the amounts of CpcG2-PBS but did not affect the protein levels of NDH-1 and PSI. Thus, we will first verify the dynamic relationship regarding the effects of light quality on NDH-1-PSI and its members, NDH-1, CpcG2-PBS and PSI; consequently, we will reveal the forming mechanism of NDH-1-PSI under stress conditions of light quality. Further, we will clarify the physiological function of NDH-1-PSI under conditions of different qualities of light. Next, we will analyze the dynamic relationship regarding the effects of other stresses on NDH-1-PSI and its members, find out the key member for the formation of NDH-1-PSI, and reveal the forming mechanism of NDH-1-PSI. Finally, we will clarify the physiological function of NDH-1-PSI under conditions of other stresses using strategies of molecular biology, plant physiology and etc. These results will certainly increase our understanding the important role of cyanobacterial NDH-1-PSI supercomplex under stress conditions and provide an important the theory reference for understanding the forming mechanism and physiological function of NDH-1-PSI in higher plants.

蓝藻NDH-1能通过CpcG2-PBS与PSI形成超分子复合物NDH-1-PSI，并专一地参与围绕PSI的环式电子传递。然而，NDH-1-PSI在胁迫条件下的形成机制及其生理功能均尚不清楚。最近，我们的初步结果指出光质（绿光和红光）能够改变CpcG2-PBS和NDH-1-PSI的数量但不影响NDH-1和PSI的数量。因此，本项目将首先证实光质对NDH-1-PSI及其组分的动态关系，进而揭示出NDH-1-PSI在光质胁迫条件下的形成机制及其生理功能。然后，通过分子和生理生化等手段分析其他胁迫条件下NDH-1-PSI及其组分的动态关系，找出影响NDH-1-PSI形成的关键组分，进而揭示出NDH-1-PSI在其他胁迫条件下的形成机制及其生理功能。这些研究结果必将增进人们对蓝藻NDH-1-PSI在胁迫条件下重要角色的理解，也为研究高等植物NDH-1-PSI的形成机制和生理功能提供重要的理论参考。

蓝藻NDH-1能通过CpcG2-PBS与PSI形成超分子复合物NDH-1-PSI，并专一地参与围绕PSI的环式电子传递（NDH-CET）。然而，NDH-1-PSI在胁迫条件下的形成机制及其生理功能均尚不清楚。我们通过蓝绿温和凝胶电泳（BN-PAGE）和蛋白免疫印迹等手段，在绿光和红光（对照：红光，为绿光的互补光）等生长条件下，分析NDH-1-PSI，以及它的组分NDH-1，CpcG2-PBS和PSI的亚基数量变化情况，发现绿光能诱导上调NDH-1-PSI及其组分CpcG2-PBS的亚基数量，但不影响该超分子复合体的其他组分NDH-1和PSI的亚基数量。因此，我们认为绿光通过增加CpcG2-PBS的量，增强了NDH-1与PSI的连接，使得NDH-1-PSI的数量增加，从而使NDH-1和PSI活性上调，加快了NDH-CET。然后，通过分子和生理生化等手段分析低碳等其他胁迫条件下NDH-1-PSI及其组分的动态关系，发现低碳胁迫下通过上调NDH-1的量来增加NDH-1-PSI的量，从而增加NDH-1活性。这些研究结果增进人们对蓝藻NDH-1-PSI在胁迫条件下重要角色的理解，也为研究高等植物NDH-1-PSI的形成机制和生理功能提供了重要的理论参考。

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