胚胎干细胞特异性miR-302-367簇诱导人宫颈癌细胞系的重编程

iPS cells are derived from somatic cells that regain pluripotency by the nuclear programming with exogenous factors. Cancer cell reprogramming is better for establishing tumor models, uncovering the pathogenesis of tumor and exploring new therapeutic targets. So far, Skin cancer, melanoma and liver cancer cells had been successfully reprogrammed into iPS cells, but the cervical cancer is still blank. Our preliminary findings show that miR-302-367 inhibits the growth of cervical cancer cells by bloking the cell cycle, surprisingly, only changing the culture conditions, miR-302-367 makes the cervical cancer cells forming a dense tumor spheres, and there is no obvious boundary between cells, which is similar to embryonic stem cell-like phenotype.These results not only show that miR-302-367 is able to induce iPS cells, but also the reprogramming of cervical cancer cells depends on the microenvironment. Therefore, this study aims to investigate the conditions of reprogramming cervical cancer by miR-302-367, and reveal the molecular mechanism, which will be used to better understand the pathogenesis of cervical cancer and supply giant cell resources for drug evaluation.

iPS细胞是成体细胞在外源因子作用下，经直接细胞核程序重整而重新获取多潜能性的干细胞。诱导癌细胞重编程有助于建立肿瘤疾病模型，揭示肿瘤的发生机制及探寻新的治疗靶点。至今为止，已成功诱导皮肤癌、黑色素瘤及肝癌细胞重编程为多能干细胞，而宫颈癌细胞重编程研究仍是空白。本课题组前期研究结果发现胚胎特异性miR-302-367通过阻滞细胞周期的进程抑制宫颈癌细胞的生长，意外的是，仅仅更换培养条件，该簇miRNAs可使宫颈癌细胞形成致密的肿瘤球，且细胞界限不明显，呈现类似于胚胎干细胞样表型，这样的结果不仅说明miR-302-367具有诱导iPS的潜能，而且说明宫颈癌细胞重编程对于微环境的依赖性。因此，本课题旨在探讨miR-302-367诱导宫颈癌iPS的条件，阐明miR-302-367重编程宫颈癌细胞的分子机制，为更好地理解宫颈癌的发病机制及药物安全性评价提供巨大细胞资源。

内容获取失败，请点击重试