剂量敏感的荧光宽色度演变试纸对重金属离子的可视化定量分析

Fluorescent test paper with the advantages of rapid, portable, low cost, visualization, has widely application value in the fields of environment, medicine, food and other detections. However, the current fluorescent test paper widespread the deficiency of narrow spectral range, low color resolution, and poor quantitative ability, and difficult to achieve the visual quantitative analysis of similar pH test paper. Here, the project will be designed with combination of fluorescence colorimetric analysis and sensitive probe design and Multi color probe to develop dosage-sensitive fluorescent color test paper, for quantitative analysis of rapid and simple visualization of heavy metal ions: (1) explore the methods and principles for the surface functionalization and specific ligands of multi colored fluorescent nanoparticles, to prepare the wide spectrum emission nanoprobe based on overcoming the overlap od spectral interference; (2) elucidate the interaction between heavy metal ions and probes, and induce the interaction between the color and intensity radio of probe fluorescence, establish the signal reading and analysis principle based on the dosage-sensitive wide spectrum fluorescence evolution technique; (3) utilize fluorescent nano probe ink to print high quality and ultra sensitive fluorescent paper, realize the quantitative analysis of heavy metal ions based on multi color evolution mode and chroma analysis. The expected results of this project will provide a new way of thinking and technical means for the development of quantitative fluorescent test paper analysis method.

荧光试纸凭借快速便携、低成本、可视化等优点，在环境、医学、食品等检测领域有广泛应用价值。然而，目前的荧光试纸普遍存在光谱变化范围窄、色度分辨低、定量能力差等不足，难以实现类似pH试纸的可视化定量分析。本项目将通过荧光色度分析和敏感探针设计与多颜色探针的组合，发展剂量敏感的荧光变色试纸，实现对重金属离子快速简便的可视化定量分析：（1）探索多颜色荧光纳米粒子表面功能化、特异性配体的组装方法和原理，在克服光谱干扰重叠基础上制备出具有宽光谱发射的纳米探针；（2）阐明重金属离子和探针之间的相互作用、及其诱导探针荧光的颜色和强度比值改变的相互关系，建立基于剂量敏感的宽光谱荧光演变技术的信号读取与分析原理；（3）利用荧光纳米探针墨水组装印刷高质量、超灵敏的荧光试纸，实现基于多色演变模式和色度分析的重金属离子可视化定量分析。本项目的预期成果将为发展可视化定量荧光试纸分析方法提供新的思路和技术手段。

本项目针对针对环境污染物（重金属离子、农残、有机污染物等）的高效富集和快速检测，开展了新型光效应增强纳米结构的设计、合成和表征等有关研究工作，探索了目标分子在纳米结构表面的识别、传输和结合动力学特征；阐明了纳米结构与敏感性和选择性相互关系的规律；发展基于纳米粒子光效应的传感分析方法，建立基于纳米光效应传感技术的高通量同步分析与探测原理；特别是，在敏感纳米探针设计与环境检测方面，制备了多种荧光响应的纳米探针。在此基础上，制备了可视化检测试纸，并结合智能手机及便携式检测仪构筑了快速便携一体化检测系统，实现了ppb量级污染物残留的快速检测。上述研究工作取得了多项具有创新性和系统性的研究成果，在高影响力的国际期刊Anal. Chem.、ACS AMI等上发表SCI论文9篇；申请国家发明专利6件；设计及开发了环境快速检测与动态监测装备系列样机两台，培养研究生3人。这些研究成果推动了我国在环境污染物的快速敏感分析检测领域与国际先进技术接轨，并为发展荧光试纸在环境、医药、食品、疾病诊断及公共安全等领域中的应用提供了新的思路和方法。

内容获取失败，请点击重试