内部萃取电喷雾电离质谱快速鉴别癌变组织边界的方法研究

Accurate surgical resection of tumor margins is a great challenge in clinical practice. There is currently a lack of analytical methods which allow rapid and accurate analysis for distinguish the tumor tissue. In our earlier collaborative study with the Department of Cardiothoracic Surgery at the Second Affiliated Hospital of Nanchang University, we have demonstrated a principal feasibility for the rapid differentiation of cancer and normal tissues using the direct ambient ionization mass spectrometry (AMS) analysis of biopsy samples. Based on the achieved results, in this project we will explore the potential of AMS analysis for the accurate identification of tumor edges under intraoperative conditions in real time using a novel AMS method called internal extractive electrospray ionization mass spectrometry (iEESI-MS). Here a stable iEESI ionization source will be developed and coupled to the LTQ-MS. A new approach iEESI-MS for acquiring the internal information of tissue sample will be proposed and this iEESI-MS will be applied to analyze the tumor and normal tissues of 100 patients. Finally, iEESI-MS will be used to identify the tumor margins in the surgical-resection. The proposed project will be accomplished within four years. In perspective, the project provides a new diagnose method of the identification of accurate tumor margins and lay the solid foundation for finding the tumor bio-marker.

癌变组织边界的快速准确鉴定是恶性肿瘤手术治疗时确定切除范围的关键前提。本项目围绕临床中难以快速准确判定恶性肿瘤癌变组织边界这一重大难题，在前期工作的基础上，针对癌症组织样本的特殊性和复杂性，开发专门的内部萃取电喷雾电离技术（iEESI）及装置，构建、优化及表征用于快速判断癌变组织是否切除完全的内部萃取电喷雾电离线性离子阱质谱（iEESI-LTQ-MS）研究平台，获得癌变组织与正常组织在分子层次的差异，并结合化学计量学方法对获得的癌症与癌旁组织的iEESI-MS数据进行区分，建立癌变组织的快速质谱鉴定研究方法，为确定临床常见恶性肿瘤（如肺癌、肝癌、食管癌等）合理的手术切除范围提供一种新的快速质谱诊断方法。