GBP介导的粘虫密度依赖性多型性状机制研究

The project aims to study the mechanism of growth blocking peptide modulated density-dependent polymorphism in the oriental armyworm Mythimna separata, to acquire new knowledge in terms of cuticle melanism, the immune defense and developmental regulation, to establish research model of density-dependent behavioral traits and to elucidate the role of GBP in regulating the density-dependent behaviors. The study includes: (1) Study the mechanism of density-dependent polymorphism induced by different densities and ways of stimulation (2) By measuring levels of GBP & juvenile hormone, doba-dopamine pathway as well as by using topical application of GBP and RNAi technique, the regulation mode and transduction pathway of GBP in the density-dependent polymorphism are studied (3) Study the behavioral traits of gregarious-solitary, feeding and cannibalism through behavior tracking and motion analysis system and establish the density-dependent behavior mode mediated by GBP (4) Study the density-dependent behavior of cannibalism under natural ecosystems through field investigation and control experiments, to explore the ecological regulation of density-dependent polymorphism and to provide a theoretical basis for the timely forecasting of the armyworm population.

本项目以迁飞性昆虫粘虫为对象，研究生长阻滞肽(GBP)介导的密度依赖性多型性状始发机制，在与密度依赖的表皮黑色素化、免疫防御能力和发育调控方面获得新知识；建立密度依赖的行为性状研究模式，阐明GBP在密度依赖行为性状中的作用和介导方式。研究内容包括：(1) 研究不同密度、不同刺激方式诱发的多型性状始发机制;(2) 通过测定GBP&保幼激素、多巴-多巴胺通路表达水平，结合局部注射GBP和RNAi沉默，研究GBP在粘虫密度依赖的多型性状中调控方式和传递路径；(3) 通过动物行为追踪和动作分析系统，开展粘虫密度依赖的群居-散居、取食和同类相食行为性状研究，建立GBP介导的密度依赖性行为性状模式；(4) 通过野外调查和控制实验，研究自然生态下密度依赖的同类相食行为，探索密度依赖的多型性状自然生态调控方式，为粘虫的适时预测预报提供理论依据。