碱性电解水缓和热应激蛋鸡氧化应激效应及细胞信号转导机制研究

Antioxidant supplementation by dietary is the important way to counteract oxidative Stress induced from heat stress for laying hen. However, with the decrease of the feed intake resulted from heat stress，antioxidant therapies by dietary do not have a satisfactory results. Drinking electrochemically reduced water (ERW) may be a better way to counteract oxidative stress induced from heat stress for laying hens. However, its effectiveness and mechanisms are largerly unknown. Our previous study found that ERW plays a important role in alleviation of lipid peroxidation for laying hens. Several findings have indicated that ERW had the ability of a scavenger free radical, and may participate in the redox regulation of cellular function, but the mechanisms are also not clear. Therefore, we hypothesize that ERW can be drunk by laying hens for relaxation oxidative stress under circumstance of heat stress, may be mainly through 2 ways: one is through inhibiting NF-κB/iNOS pathway to protect mitochondria, another is inhibition of JNK and p53 activation to prevent subsequent apoptosis prone signals activation. To test this hypothesis, we study the effective of drinking ERW by laying hens to reduce oxidative stress resulted from heat stress in animal and cell level, RT-PCR, western blot and electron microscopy technology will be used to study the resistance mechanism of cell apoptosis from oxidative stress. This research will provide new idea for heat stress prevention of laying hens.

饲用抗氧化剂可缓和热应激给蛋鸡生产带来的重大损失，但该方式因热应激采食量下降而效果不佳，饮用抗氧化剂更利于缓和热应激，但用量大成本高。碱性电解水（Electrochemically Reduced water，ERW）是一种可清除机体自由基的抗氧化饮用水，价廉高效且制作简单，却从未在蛋鸡中应用，效果和机制不详；我们研究发现ERW可用于蛋鸡饮用，再结合相关ERW抗氧化应激细胞凋亡作用的研究报道，我们推测ERW可用于缓和热应激蛋鸡的氧化应激，且主要可能通过两条路径，一是NF-κB/iNOS途径缓解线粒体膜损伤，二是抑制JNK及p53通路抗细胞凋亡。为证实该假说，我们在整体和细胞两个水平探讨饮用ERW对热应激蛋鸡氧化应激的缓和效应，并用RT-PCR 及Western blot、荧光聚焦扫描电镜等技术研究其抗氧化应激细胞凋亡机制。此研究将为蛋鸡热应激防治提供新思路和新靶点。

饲用抗氧化剂可缓和热应激给蛋鸡生产带来的重大损失，但该方式因热应激采食量下降而效果不佳，饮用抗氧化剂更利于缓和热应激，但用量大成本高。碱性电解水（Electrolyzed Reduce water，ERW）是一种可清除机体自由基的抗氧化饮用水，价廉高效且制作简单，却从未在蛋鸡中应用，效果和机制不详；我们研究发现ERW可用于蛋鸡饮用，再结合相关ERW抗氧化应激细胞凋亡作用的研究报道，我们推测ERW可用于缓和热应激蛋鸡的氧化应激，且主要可能通过两条路径，一是NF-κB/iNOS途径缓解线粒体膜损伤，二是抑制JNK及p53通路抗细胞凋亡。为证实该假说，我们在整体和细胞两个水平探讨饮用ERW对热应激蛋鸡氧化应激的缓和效应，并用RT-PCR及Western-blot等技术研究其抗氧化应激细胞凋亡机制。研究结果表明ERW可缓和热应激蛋鸡的氧化应激，尤其可缓解热应激蛋鸡的肠道氧化损伤。研究阐明了ERW可通过抑制NF-κB /iNOS途径抗 H2O2 对蛋鸡原代肝细胞的线粒体损伤及通过抑制JNK及p53通路抗H2O2诱导的细胞凋亡。此研究将为蛋鸡热应激防治提供新思路和新靶点。

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