死后尸体组织红外显微光谱及Mapping图像时序性变化推断死亡时间的研究

It is significant complicated and difficult to determine the postmortem interval. The environment conditions and death causes are important factors to influence the estimation of postmortem interval. The changes of postmortem phenomena including algor mortis, livor mortis, rigor mortis,etc. tend to stable 24 hours after death. It is more difficult to determine the later postmortem interval. The changes of biomolecular functions can be showed using FTIR spectroscopy. It is important potential value to explore changes of postmortem chemistry and the relationship with postmortem interval, to clarify the influence from environment conditions and death causes, to establish the mathematical estimating models. The project wants to resolve the problems based on the previous research using FTIR microspectroscopy and Mapping technique:1. to expore postmortem infrared microspectral changes of the tissues of different organs and establish the multivariate and single factor mathematical models; 2.to clarify the microspectral changes rules in the different tissues and the relationship with postmortem inteval after death；3. to establsih the standard infrared microspectral and Mapping images atlas; 4.to analyze the effects to microspectral changes from death causes and environment conditions and verify the feasibility and accuracy of the the multivariate and single factor mathematical models.

死亡时间推断十分复杂和困难，尸体存放环境、死亡原因是干扰死亡时间推断的最大因素。死后24小时后尸冷、尸斑、尸僵等尸体现象变化将趋于稳定，故晚期死亡时间推断难度更大。FTIR光谱能有效的反映出生物大分子化学基团的变化，运用FTIR显微光谱研究死后尸体化学变化及其与死亡时间关系，并明确环境和死因对死亡时间推断的影响，构建晚期死亡时间推断数学模型具有重要的意义。本项目在前期大量研究的基础上，运用FTIR显微光谱及Mapping技术拟解决：1、研究死后不同器官各类组织的红外显微光谱变化，并根据显微光谱变化建立涉及温度和死因的多元和单因素数学模型；2、在细胞水平上阐明不同组织死后显微光谱变化规律及其与死亡时间的关系；3、构建死后不同时间的标准红外显微光谱图谱及Mapping图像图谱；4、分析死因、尸体存放环境对光谱变化的影响，并验证多元和单因素数学模型的可行性和准确性。