基于二维材料和金属纳米结构的集成微流控生化传感器研究

Because there are some difficulties for the existing sensing technology to detect the trace molecular cancer markers in early stage, we research and develop a new kind of biosensor based on two-dimensional material - metal nanostructures which is integrated with microfluidic to form LSPR - SERS "dual" sensing biochemical sensors. This kind of biosensor can meet the requirements of multi-parameters and high sensitivity detection for the cancer early markers. To design a new type of probing molecules was used to realize high specificity and high selectivity through local assembling target -biochemical molecules. To improve sensitivity was realized by using two-dimensional material cooperating with metal nanostructure plasmon. Meanwhile, time domain enhance of the biosensor came true through microfluidics. We investigate the miRNA specific capture, metal nanostructures SERS and LSPR sensing, two-dimensional material sensing mechanism, the "hot spot" produced by metal nanostructure in the process of energy transfer and key scientific problems, such as signal enhancement process for detecting low concentration of miRNA. And we eventually build detection theory framework of multiple interface among nanostructures, the probing molecules and target biological molecules. There are some innovation in the new pattern, new methods and new theory of high sensitivity two-dimensional material - metal nanostructures biosensor, micro/nano-fabrication technology of biosensor integrated with multi-channel, testing and data processing.

针对现有传感技术对微量癌症早期标志物分子检测的困难，研发基于二维材料-金属纳米结构的集成微流控LSPR-SERS“双”生化传感器，以满足癌症早期标志物分子多参数、高灵敏度检测的需求。设计新型的探针分子并进行局域组装实现生化分子的高特异性和高选择性，利用二维材料与金属增敏结构的等离激元协同增强作用提高灵敏度，通过微流控技术实现传感过程的时域增强。通过研究miRNA特异性捕捉、金属纳米结构SERS传感和LSPR传感、二维材料传感增强机制、金属纳米结构产生的“hot spot”增敏过程中的能量转移及信号增强过程等关键科学问题，实现极低浓度miRNA的检测。并最终构建纳米结构表面与探针分子、目标生物分子之间的多重界面控制的生物信息检测理论框架，在构建高增敏二维材料-金属纳米结构生物传感器新模式、新方法和新理论、多通道生物传感器的微纳加工技术、检测和数据处理技术方面获得创新成果。