Route to one-step microstructure mold fabrication for PDMS microfluidic chip

The microstructure mold fabrication for PDMS microfluidic chip remains complex and time-consuming process requiring special equipment and protocols: photolithography and etching. Thus, a rapid and cost-effective method is highly needed. Comparing with the traditional microfluidic chip fabricating process based on the micro-electromechanical system (MEMS), this method is simple and easy to implement, and the whole fabrication process only requires 1-2 h. Different size of microstructure from 100 to 1000 mu m was fabricated, and used to culture four kinds of breast cancer cell lines. Cell viability and morphology was assessed when they were cultured in the micro straight channels, micro square holes and the bonding PDMS-glass microfluidic chip. The experimental results indicate that the microfluidic chip is good and meet the experimental requirements. This method can greatly reduce the process time and cost of the microfluidic chip, and provide a simple and effective way for the structure design and in the field of biological microfabrications and microfluidic chips. (C) 2018 Author(s).

聚二甲基硅氧烷（PDMS）微流控芯片的微结构模具制造仍然是一个复杂且耗时的过程，需要特殊的设备和工艺：光刻和蚀刻。因此，非常需要一种快速且具有成本效益的方法。与基于微机电系统（MEMS）的传统微流控芯片制造工艺相比，这种方法简单易行，整个制造过程仅需1 - 2小时。制造了尺寸从100到1000微米不同的微结构，并用于培养四种乳腺癌细胞系。当细胞在微直通道、微方孔以及键合的PDMS - 玻璃微流控芯片中培养时，对细胞活力和形态进行了评估。实验结果表明，该微流控芯片良好，符合实验要求。这种方法可以大大减少微流控芯片的加工时间和成本，并为生物微制造和微流控芯片领域的结构设计提供一种简单有效的途径。（C）2018作者。