用于上皮-间充质转化研究的电化学生物传感系统的建立

结题报告

项目介绍

AI项目解读

基本信息

批准号：
31801200
项目类别：
青年科学基金项目
资助金额：
25.0万
负责人：
杜欣
依托单位：
山东师范大学
学科分类：
C0709.细胞外微环境与细胞间通讯
结题年份：
2021
批准年份：
2018
项目状态：
已结题
起止时间：
2019-01-01 至2021-12-31

项目参与者：
董喜凤；张鸿雁；冉杰；周鹏；张振华；吴雨翰；
关键词：
上皮-间充质转化生物传感器纳米复合材料量子点分子探针与标记

项目摘要

At present, there are more and more researches on cell biological processes. However, in basic or clinical research, most of the methods used in detection and analysis are still traditional methods. Most of these methods cannot monitor the cell growth process dynamically. Therefore, it is of great significance to develop a convenient and rapid method for in-situ detection in cell culture. Epithelial-mesenchymal transition (EMT) plays an important role in embryonic development, the growth of tissue and organ, and metastasis, and is therefore receiving widespread attention. This project crosses multiple disciplines and utilizes the advantages of real-time and low-cost of electrochemical biosensor technology. Moreover, EMT is used as the main research model, marker protein of EMT is used as detection target, quantum dots linked specific antibody are used as probes and the carbon nanotube-gold nanoparticle composite material is used to modify the sensing substrate. Finally, the aim of our project is to establish a novel and modern biological method suitable for three levels of analysis in cells, tissues, and clinical fields. The project is expected to provide new ideas for the development of new methods for cell activity testing, and to further advance the application of biosensor technology in biology, medicine and other fields.

目前，关于细胞生物学过程的研究越来越多，但无论是基础还是临床研究中，分析检测时多数采用的仍是传统方法，这些方法大部分都不能对细胞的生长过程做出动态监测和分析，因此发展一种细胞培养过程中原位检测的、方便快捷的方法具有重要的意义。上皮-间充质转化(Epithelial-mesenchymal transition, EMT)在胚胎发育、组织、器官生长、肿瘤转移中都起到重要作用，因此受到广泛关注。本项目交叉多种学科，利用电化学生物传感技术实时性、成本低等优势，以EMT为主要研究模型，EMT标志物蛋白作为检测靶点，连接其特异性抗体的量子点作为探针，碳纳米管-金纳米颗粒复合材料修饰传感基片，最终建立适用于细胞、组织、临床三个水平分析检测的、新颖的现代生物学方法。该项目有望为发展细胞活动检测新方法提供新思路，并进一步推进生物传感技术在生物学、医学等领域上的应用。