Novel technology for quality control of viral vector particles

病毒载体颗粒质量控制新技术

基本信息

批准号：
570692-2021
负责人：
DeKoninck, YvesJG
金额：
$ 36.25万
依托单位：
Université Laval
依托单位国家：
加拿大
项目类别：
Alliance Grants
财政年份：
2022
资助国家：
加拿大
起止时间：
2022-01-01 至 2023-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=763631
关键词：
Novel technology quality control viral

项目摘要

The goal of gene therapy is to modify or manipulate the expression of a gene or to alter the biological properties of living cells for therapeutic use. Viral vectors, which are modified viruses devoid of pathogenicity, are ideal vehicles to deliver genetic material for the purpose of gene therapy. Over the last decade, significant improvements in their design and composition have enhanced safety and placed viral vector-based therapies at the forefront of modern medicine. Viral vectors have been employed for the treatment of various diseases such as metabolic, cardiovascular, muscular, hematologic, ophthalmologic, and are used as platforms for vaccine delivery. Although the manufacturing of these vectors has also progressed, increases in demand now requires significant efforts to increase yield and purity. This project proposes to exploit the technology developed by Affinité Instruments, a Canadian company, to develop new and affordable quality control strategies to monitor the efficiency of viral vector production during the manufacturing process. As communicated through the recent "Canada's Biomanufacturing and Life Sciences Strategy" Canada is in desperate need to re-instate a flexible biomanufacturing capacity to respond to unexpected challenges such as the one we are facing with COVID-19. The technologies developed in the context of the proposed project will contribute to the collective efforts to rebuild a strong, efficient, and resilient biomanufacturing capacity in Canada.

基因治疗的目标是修饰或操纵基因的表达或改变活细胞的生物学特性以用于治疗用途。病毒载体是缺乏致病性的修饰病毒，是传递遗传物质的理想载体。在过去的十年中，其设计和成分的显着改进提高了安全性，并且基于病毒载体的疗法处于现代医学的前沿，已用于治疗各种疾病，例如代谢、心血管、肌肉疾病。 , 血液学,尽管这些载体的制造也取得了进展，但现在需求的增加需要付出巨大努力来提高产量和纯度。该项目建议利用加拿大公司 Affinité Instruments 开发的技术，制定新的、负担得起的质量控制策略，以监测制造过程中病毒载体生产的效率，正如最近的“加拿大生物制造和生命科学战略”所传达的那样，加拿大迫切需要恢复灵活的生产流程。生物制造能力以应对意外挑战，例如我们面临的 COVID-19 挑战。在拟议项目中开发的技术将有助于共同努力，重建加拿大强大、高效和有弹性的生物制造能力。