Non-releasing alginate hydrogels for analyte sensing and detoxification

用于分析物传感和解毒的非释放藻酸盐水凝胶

基本信息

批准号：
RGPIN-2022-04384
负责人：
Matoori, Simon
金额：
$ 1.89万
依托单位：
Université de Montréal
依托单位国家：
加拿大
项目类别：
Discovery Grants Program - Individual
财政年份：
2022
资助国家：
加拿大
起止时间：
2022-01-01 至 2023-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=762037
关键词：
Non releasing alginate hydrogels analyte

项目摘要

Alginate hydrogels are widely used in tissue engineering, drug delivery, and wound healing applications because of their high biocompatibility, low toxicity, and ease of gelation. In this research program, we propose to develop novel non-releasing alginate hydrogels for analyte sensing and detoxification. In these applications, materials with high ligand densities and low ligand release rates are needed, as the detection or detoxification occurs inside the material. Indeed, ligand release reduces the activity of the system and may lead to toxicity after local or systemic exposure to the ligand. Small molecules and proteins are generally released very rapidly from hydrogels. Therefore, new strategies for the development of non-releasing hydrogels for these applications are needed. I propose to use a covalent conjugation approach to load alginate gels with bioactive cargo and test the hydrogels as wound dressings for chronic wounds. Chronic wounds can have several origins but share key features, namely high reactive oxygen species (ROS) and high matrix metalloproteinase 9 levels, and abnormal pH. These new hydrogels are highly versatile and promise to impact the biotechnology landscape of Canada based on their capacity to lead to patent applications with high licensing potential and/or the foundation of startups.

海藻酸盐水凝胶因其高生物相容性、低毒性和易于凝胶化而广泛应用于组织工程、药物输送和伤口愈合应用。在该研究计划中，我们建议开发新型非释放藻酸盐水凝胶，用于分析物传感和解毒。在这些应用中，需要具有高配体密度和低配体释放速率的材料，因为检测或解毒发生在材料内部。事实上，配体释放会降低系统的活性，并可能在局部或全身暴露于配体后导致毒性。小分子和蛋白质通常从水凝胶中快速释放。因此，需要开发用于这些应用的非释放水凝胶的新策略。我建议使用共价缀合方法将生物活性物质负载到藻酸盐凝胶中，并测试水凝胶作为慢性伤口的伤口敷料。慢性伤口可能有多种起源，但有一些共同的关键特征，即高活性氧 (ROS) 和高基质金属蛋白酶 9 水平以及 pH 值异常。这些新型水凝胶用途广泛，有望影响加拿大的生物技术格局，因为它们能够带来具有高许可潜力的专利申请和/或初创公司的基础。