Detoxification functionality of lactic acid bacteria
乳酸菌的解毒功能
基本信息
- 批准号:RGPIN-2019-04028
- 负责人:
- 金额:$ 2.58万
- 依托单位:
- 依托单位国家:加拿大
- 项目类别:Discovery Grants Program - Individual
- 财政年份:2022
- 资助国家:加拿大
- 起止时间:2022-01-01 至 2023-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Having shown in our current NSERC Discovery Grant that lactobacilli can perform detoxification of heavy metals, aflatoxins and pesticides, we now wish to identify the mechanisms involved in mitigating the pesticide effects. This is important to counter colony collapse disorder in honey bees. Nothing endangers the global food supply more than the loss of these pollinators. Our preliminary findings suggest that commonly used pesticides impair insect immunity making them more susceptible to a variety of pathogens. Hypothesis We hypothesize that members of the genus Lactobacillus harbor the ability to detoxify pesticides that endanger honey bees and other life forms. Long term goals The long-term goal of this research program is to advance our knowledge of an important bacterial genus with a long history of safe use in foods and supplements, and to exploit attributes that counter environmental chemicals and improve the survival of pollinators. Research objectives 1.1.Characterize how lactobacilli mitigates the immunosuppressive effects of pesticides and susceptibility to pathogens 2.Determine the functional capacity of lactobacilli to detoxify agriculturally-relevant xenobiotics. Methodological approach We will use Drosophila melanogaster as a well-established and strongly predictive model of the honey bee to determine how lactobacilli affect the co-modulatory response of innate immunity (highly conserved across broad taxonomic range) towards pesticides and/or pathogens as they apply primarily to honeybees, but also other hosts. We will utilize our stocks of RNAi-mediated knockdown and overexpression mutants of the DUOX and Imd pathways, and couple these knockouts with qPCR-based measurement of Imd and DUOX pathway-related gene expression and immunofluorescent stain relevant immune proteins. We will expose flies to different classes of pesticides: neonicotinoids, organophosphates, carbamates, Roundup (glyphosate), acephate, ?-cyhalothrin, oxamyl, tetraconazole administered at different concentrations. Lactobacillus rhamnosus GR-1, L. plantarum Lp39, L. kunkeei BR1, L. reuteri RC-14, L. rhamnosus GG and strains from African bees will be used to counter the pesticides and determine if they can actually degrade the chemicals. We will compare the microbiota in bees from healthy hives to that of hives infected with P. larvae, Melissococcus plutonius, N. apis, N. ceranae, Ascosphaera apis, Crithidia bombi or Varroa destructor, pathogens that will be examined in vitro for the ability of the lactobacilli to inhibit them. The Drosophila model will also be used to test various lactobacilli for their ability to modulate detoxification pathways. Significance The net result will be to develop an understanding of how lactobacilli strains can counter the most widely used pesticides through their degradation, and by modulation of insect immune responses, microbiota resilience, and countering key honey bee pathogens.
我们目前的 NSERC 发现资助表明,乳酸杆菌可以对重金属、黄曲霉毒素和农药进行解毒,现在我们希望确定减轻农药影响所涉及的机制。这对于对抗蜜蜂的蜂群崩溃失调非常重要。没有什么比这些授粉媒介的消失对全球粮食供应的危害更大了。我们的初步研究结果表明,常用的杀虫剂会损害昆虫的免疫力,使它们更容易受到各种病原体的影响。 假设 我们假设乳酸菌属的成员具有解毒杀虫剂的能力,这些杀虫剂会危及蜜蜂和其他生命形式。 长期目标 该研究计划的长期目标是增进我们对重要细菌属的了解,该细菌属在食品和补充剂中具有悠久的安全使用历史,并利用对抗环境化学物质的特性并提高传粉媒介的生存率。 研究目标 1.1. 表征乳酸菌如何减轻农药的免疫抑制作用和对病原体的敏感性 2. 确定乳酸菌解毒农业相关外源物质的功能能力。方法 我们将使用黑腹果蝇作为蜜蜂的成熟且强有力的预测模型,以确定乳酸杆菌如何影响对农药和/或病原体应用时的先天免疫(在广泛的分类范围内高度保守)的共调节反应主要针对蜜蜂,但也针对其他宿主。我们将利用我们的 DUOX 和 Imd 途径的 RNAi 介导的敲低和过度表达突变体库存,并将这些敲除与基于 qPCR 的 Imd 和 DUOX 途径相关基因表达和免疫荧光染色相关免疫蛋白的测量结合起来。我们将让苍蝇接触不同类别的农药:新烟碱类、有机磷酸酯、氨基甲酸酯、农达(草甘膦)、乙酰甲胺磷、β-三氯氟氰菊酯、草酰草酯、四康唑,并以不同浓度施用。鼠李糖乳杆菌 GR-1、植物乳杆菌 Lp39、昆基乳杆菌 BR1、罗伊氏乳杆菌 RC-14、鼠李糖乳杆菌 GG 和来自非洲蜜蜂的菌株将用于对抗农药,并确定它们是否能够真正降解化学物质。我们将比较健康蜂巢中蜜蜂的微生物群与被 P. larvae、Melissococcus plutonius、N. apis、N. ceranae、Ascosphaera apis、Critithiaombi 或 Varroa destructor 感染的蜂巢的微生物群,这些病原体将在体外检查其能力的乳酸杆菌来抑制它们。果蝇模型还将用于测试各种乳酸杆菌调节解毒途径的能力。意义 最终的结果将是加深对乳酸杆菌菌株如何通过降解、调节昆虫免疫反应、微生物群恢复能力和对抗关键蜜蜂病原体来抵抗最广泛使用的农药的了解。
项目成果
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Reid, Gregor其他文献
Is bacterial vaginosis a disease?
- DOI:
10.1007/s00253-017-8659-9 - 发表时间:
2018-01-01 - 期刊:
- 影响因子:5
- 作者:
Reid, Gregor - 通讯作者:
Reid, Gregor
The Rationale for Probiotics Improving Reproductive Health and Pregnancy Outcome
- DOI:
10.1111/aji.12086 - 发表时间:
2013-06-01 - 期刊:
- 影响因子:3.6
- 作者:
Reid, Jennifer N. S.;Bisanz, Jordan E.;Reid, Gregor - 通讯作者:
Reid, Gregor
PCR-based identification of eight lactobacillus species and 18 hr-HPV genotypes in fixed cervical samples of south african women at risk of HIV and BV
- DOI:
10.1002/dc.21786 - 发表时间:
2012-06-01 - 期刊:
- 影响因子:1.3
- 作者:
Dols, Joke A. M.;Reid, Gregor;Boon, Mathilde E. - 通讯作者:
Boon, Mathilde E.
sequence tag and primer sequences.
- DOI:
10.1371/journal.pone.0015406.t002 - 发表时间:
2010-01-01 - 期刊:
- 影响因子:0
- 作者:
Macklaim, Jean M;Reid, Gregor;Hummelen, Ruben - 通讯作者:
Hummelen, Ruben
Improved cure of bacterial vaginosis with single dose of tinidazole (2 g), Lactobacillus rhamnosus GR-1, and Lactobacillus reuteri RC-14: a randomized, double-blind, placebo-controlled trial
- DOI:
10.1139/w08-102 - 发表时间:
2009-02-01 - 期刊:
- 影响因子:2.8
- 作者:
Martinez, Rafael C. R.;Franceschini, Silvio A.;Reid, Gregor - 通讯作者:
Reid, Gregor
Reid, Gregor的其他文献
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{{ truncateString('Reid, Gregor', 18)}}的其他基金
Detoxification functionality of lactic acid bacteria
乳酸菌的解毒功能
- 批准号:
RGPIN-2019-04028 - 财政年份:2021
- 资助金额:
$ 2.58万 - 项目类别:
Discovery Grants Program - Individual
Detoxification functionality of lactic acid bacteria
乳酸菌的解毒功能
- 批准号:
RGPIN-2019-04028 - 财政年份:2020
- 资助金额:
$ 2.58万 - 项目类别:
Discovery Grants Program - Individual
Detoxification functionality of lactic acid bacteria
乳酸菌的解毒功能
- 批准号:
RGPIN-2019-04028 - 财政年份:2019
- 资助金额:
$ 2.58万 - 项目类别:
Discovery Grants Program - Individual
Probiotic mechanisms of Lactobacillus in metal detoxification
乳酸菌在金属解毒中的益生机制
- 批准号:
RGPIN-2014-05188 - 财政年份:2018
- 资助金额:
$ 2.58万 - 项目类别:
Discovery Grants Program - Individual
Microbiological Food Safety in Fermented Foods
发酵食品中的微生物食品安全
- 批准号:
521893-2017 - 财政年份:2017
- 资助金额:
$ 2.58万 - 项目类别:
Engage Grants Program
Probiotic mechanisms of Lactobacillus in metal detoxification
乳酸菌在金属解毒中的益生机制
- 批准号:
RGPIN-2014-05188 - 财政年份:2017
- 资助金额:
$ 2.58万 - 项目类别:
Discovery Grants Program - Individual
Probiotic mechanisms of Lactobacillus in metal detoxification
乳酸菌在金属解毒中的益生机制
- 批准号:
RGPIN-2014-05188 - 财政年份:2016
- 资助金额:
$ 2.58万 - 项目类别:
Discovery Grants Program - Individual
Probiotic mechanisms of Lactobacillus in metal detoxification
乳酸菌在金属解毒中的益生机制
- 批准号:
RGPIN-2014-05188 - 财政年份:2015
- 资助金额:
$ 2.58万 - 项目类别:
Discovery Grants Program - Individual
Climate Change and Aquaculture in Canada: Atlantic Workshop
加拿大的气候变化和水产养殖:大西洋研讨会
- 批准号:
477446-2014 - 财政年份:2014
- 资助金额:
$ 2.58万 - 项目类别:
Strategic Workshops Program
Probiotic mechanisms of Lactobacillus in metal detoxification
乳酸菌在金属解毒中的益生机制
- 批准号:
RGPIN-2014-05188 - 财政年份:2014
- 资助金额:
$ 2.58万 - 项目类别:
Discovery Grants Program - Individual
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Detoxification functionality of lactic acid bacteria
乳酸菌的解毒功能
- 批准号:
RGPIN-2019-04028 - 财政年份:2021
- 资助金额:
$ 2.58万 - 项目类别:
Discovery Grants Program - Individual
Detoxification functionality of lactic acid bacteria
乳酸菌的解毒功能
- 批准号:
RGPIN-2019-04028 - 财政年份:2020
- 资助金额:
$ 2.58万 - 项目类别:
Discovery Grants Program - Individual
Detoxification functionality of lactic acid bacteria
乳酸菌的解毒功能
- 批准号:
RGPIN-2019-04028 - 财政年份:2019
- 资助金额:
$ 2.58万 - 项目类别:
Discovery Grants Program - Individual
Regulation of lactic acid bacteria-derived membrane vesicle production with immunostimulatory activity and their functionality design
具有免疫刺激活性的乳酸菌来源的膜囊泡产生的调节及其功能设计
- 批准号:
18K04857 - 财政年份:2018
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Elucidation of the effects of lactic acid bacteria derived from plant on functionality of phytoestrogen-metabolizing bacteria
阐明植物来源的乳酸菌对植物雌激素代谢菌功能的影响
- 批准号:
15K07450 - 财政年份:2015
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