Bee vectored Clonostachys rosea interactions with three pathogens in high bush blueberries and development of a rapid molecular detection tool

蜜蜂媒介的红罗竹与高丛蓝莓中三种病原体的相互作用以及快速分子检测工具的开发

• 批准号: 561181-2020
• 负责人: Henderson, DeborahDE
• 金额: $ 5.46万
• 依托单位: Kwantlen Polytechnic University
• 依托单位国家: 加拿大
• 项目类别: Applied Research and Development Grants - Level 2
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=755492
• 关键词: Bee; vectored; Clonostachys; rosea; interactions

The Institute for Sustainable Horticulture (ISH) at Kwantlen Polytechnic University and Bee Vectoring Technology International Inc. (BVT), a Canadian bio-pesticide company, will partner to explore the value of a bee vectored beneficial endophytic fungus, Clonostachys rosea (strain CR7) for the management of three pathogens that infect flowers of highbush blueberry in British Columbia; Mummy Berry, Monilinia vaccinii-corymbosi, Grey Rot, Botrytis cinerea, and Anthracnose Colletotrichum acutatum. The unique action of this fungus is that it establishes in plant tissue and can prevent pathogenic fungi from initiating infection. The highly targeted method of application, using pollinators to deliver the beneficial fungus to flowers, is also novel and if successful, could contribute to replacing use of chemical fungicides with an environmentally safe alternative. BVT will use the data to register the new biofungicide in Canada and make it available to the market. Benefits will also accrue to agricultural producers when this product becomes available, as it will reduce the use of fungicides, lessen heavy equipment damage to soils and lower farm staff time required to manage these diseases; ultimately lowering the cost of agricultural production while producing safer healthier blueberries. Research will study the behaviour, persistence and migration of the endophytic fungus within the blueberry plant using specific molecular tools developed in this project. Ultimately, this research will bring new, environmentally protective products for disease management to the important BC blueberry industry, train several students and new grads and a Post Doctoral Fellow in the development and use of "green" technologies, and assist BVT to become a successful company in the biopesticide sector. The field of applied research in microbial biocontrol will be advanced and KPU will expand its expertise and ability to partner with companies in this rapidly changing technology sector.

昆特兰理工大学可持续园艺研究所 (ISH) 和加拿大生物农药公司 Bee Vectoring Technology International Inc. (BVT) 将合作探索蜂媒有益内生真菌 Clonostachysrosea（CR7 菌株）的价值用于控制感染不列颠哥伦比亚省高丛蓝莓花朵的三种病原体；木乃伊浆果、Monilinia vaccinii-corymbosi、灰腐病、灰葡萄孢和炭疽病炭疽病。 这种真菌的独特作用是它在植物组织中建立并可以防止病原真菌引发感染。使用传粉媒介将有益真菌传递给花朵的高度针对性的施用方法也是新颖的，如果成功，可能有助于用环境安全的替代品取代化学杀菌剂的使用。 BVT 将利用这些数据在加拿大注册新型生物杀菌剂并将其推向市场。当该产品上市时，农业生产者也将受益，因为它将减少杀菌剂的使用，减少重型设备对土壤的损害，并减少农场工作人员管理这些疾病所需的时间；最终降低农业生产成本，同时生产出更安全、更健康的蓝莓。 研究将使用本项目开发的特定分子工具来研究蓝莓植物内内生真菌的行为、持久性和迁移。最终，这项研究将为重要的 BC 蓝莓产业带来用于疾病管理的新型环保产品，培训数名学生和应届毕业生以及一名博士后研究员开发和使用“绿色”技术，并协助 BVT 成为成功的公司属于生物农药领域。微生物生物防治的应用研究领域将得到发展，KPU 将扩大其专业知识和与这个快速变化的技术领域的公司合作的能力。