Study on the viscoelastic properties of gels from the extra-cellular matrix of decellularized tissue for therapeutic applications in cardiac and parenchymal tissue

研究脱细胞组织细胞外基质凝胶的粘弹性特性，用于心脏和实质组织的治疗应用

• 批准号: 578501-2022
• 负责人: Mantovani, DiegoD
• 金额: $ 3.64万
• 依托单位: Université Laval
• 依托单位国家: 加拿大
• 项目类别: Alliance Grants
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=753943
• 关键词: Study; viscoelastic; properties; gels; extra

The field of health, including surgery and regenerative medicine, requires materials allowing the migration, retention and growth of healthy cells from the surrounding tissues of the patient. In the form of gels and hydrogels, these materials are considered scaffold structures. They can be injected directly (without surgery) into the pathological site requiring treatment, or molded or printed, and then implanted using conventional surgery. In any case, the elastic and viscous properties of these highly hydrated materials are essential in order to modulate the gelation and the biological properties. Currently, the ability to cellularize these materials, by integrating cells, is the next frontier in this field. The characterization of cellularized samples would make it possible to develop gels which, when implanted, would be able to regenerate and replace the functions of diseased tissues. In the proposed project, the first objective consists in the development of a novel sample holder for the contactless viscoelastic characterization of cellularized hydrogels to be used with the innovative and unique theological equipment developed by Rheolution. Second, thanks to the presence of Tissuegraft, and their expertise in formulating hydrogels from decellularized extracellular matrix extracted from animal tissues, will be the optimization of the composition of a gel stimulating recruitment and regeneration from human cells for the repair and regeneration of defects in cardiac or parenchymal organs, using the sample holder developed by Rheolution and validated within the framework of the 1st objective. The unique expertise at the Biomaterials and Bioengineering Laboratory of ULaval, combining the modification of surface properties and the mechanical characterization of the gels developed, constitutes the keystone to allow the characterization of these cellularized gels. Finally, the third objective is to explore the potential for the development of a new in vitro model based on the developed Rheolution sample holder and Tissuegraft hydrogels.

健康领域，包括手术和再生医学，需要材料，允许健康细胞从患者周围组织中迁移，保留和生长。以凝胶和水凝胶的形式，这些材料被认为是支架结构。可以将它们直接（无需手术）注射到需要治疗，模制或印刷的病理部位，然后使用常规手术植入。在任何情况下，这些高度水合的材料的弹性和粘性特性对于调节凝胶化和生物学特性至关重要。当前，通过整合细胞来使这些材料的细胞化能力是该领域的下一个领域。细胞化样品的表征将有可能发展凝胶，植入后能够再生并替代患病组织的功能。在拟议的项目中，第一个目标是开发一种新型样品持有者，用于将细胞化水凝胶的非接触式粘弹性表征与Rheolution开发的创新和独特的神学设备一起使用。 Second, thanks to the presence of Tissuegraft, and their expertise in formulating hydrogels from decellularized extracellular matrix extracted from animal tissues, will be the optimization of the composition of a gel stimulating recruitment and regeneration from human cells for the repair and regeneration of defects in cardiac or parenchymal organs, using the sample holder developed by Rheolution and validated within the framework of the 1st objective. Ulaval的生物材料和生物工程实验室的独特专业知识，结合了表面特性的修饰和开发的凝胶的机械表征，构成了基石，允许对这些细胞化凝胶的表征进行表征。最后，第三个目标是探索基于开发的流变样品持有人和组织晶型水凝胶开发新的体外模型的潜力。