Microplate based LSPR biosensors

基于微孔板的 LSPR 生物传感器

• 批准号: 576563-2022
• 负责人: Wylie, RyanG
• 金额: $ 9.11万
• 依托单位: McMaster University
• 依托单位国家: 加拿大
• 项目类别: Idea to Innovation
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=748240
• 关键词: Microplate; based; LSPR; biosensors

Interactions between biomolecules underlie all biochemical processes and so are central to therapeutics and biotechnology. Understanding the strengths of these interactions is thus fundamental to the development of new technologies. Label-free techniques such as local surface plasmon resonance (LSPR) are the gold standard for measuring these important interactions, but access to LSPR instrumentation is limited due to expensive, specialized optical equipment. Current commercial sensors are also limited by non-specific interactions, and can't be easily used in complex environments such as blood, which further hinders utility. To overcome these two main limitations of this important technique, we have developed a new type of sensor for use in a plate reader, which is a common piece of laboratory equipment, and which uses a scalable technique to produce a coating that reduces the non-specific interactions. So far, this new and accessible LSPR platform has garnered attention from both industry leading plate reader manufacturers and therapeutic development companies. This proposal includes a work plan that will translate our proof-of-concept sensors into an automated fluid handling platform which is compatible with common plate readers and expand the chemistry of the coatings to allow the sensors to be used with a broader range of analytical targets relevant to therapeutic development. By the end of this I2I project period, we aim to develop a working platform that can be used by therapeutic developers and licensed with a manufacturing partner.

生物分子之间的相互作用是所有生化过程的基础，因此是治疗学和生物技术的核心。因此，了解这些相互作用的优势对于新技术的开发至关重要。局部表面等离子体共振 (LSPR) 等无标记技术是测量这些重要相互作用的黄金标准，但由于昂贵的专用光学设备，LSPR 仪器的使用受到限制。目前的商用传感器还受到非特异性相互作用的限制，并且不能轻易地在血液等复杂环境中使用，这进一步阻碍了实用性。为了克服这项重要技术的这两个主要限制，我们开发了一种用于读板器的新型传感器，这是一种常见的实验室设备，并且使用可扩展的技术来生产涂层，以减少非具体的相互作用。到目前为止，这个新的、易于使用的 LSPR 平台已经引起了行业领先的读板机制造商和治疗开发公司的关注。该提案包括一项工作计划，将我们的概念验证传感器转化为自动化流体处理平台，该平台与常见的读板机兼容，并扩展涂层的化学成分，使传感器能够用于更广泛的分析目标与治疗发展相关。到 I2I 项目期结束时，我们的目标是开发一个可供治疗开发商使用并获得制造合作伙伴许可的工作平台。