Structure-Property relationship of S-layer protein assemblies at extremophilic archea bacteria

极端古细菌S层蛋白组装体的结构-性质关系

• 批准号: RGPIN-2018-04970
• 负责人: Stetefeld, Jörg
• 金额: $ 6.12万
• 依托单位: University of Manitoba
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=748106
• 关键词: Structure; Property; relationship; layer; protein; Structure; Property; relationship; layer; protein

Nature of the WorkThe long term objective of my research program is to investigate the extreme stability and unique properties of archaea Surface layer (S-layer) components for the ultimate purpose to engineer nano-devices in a variety of biotechnological fields. The research program combines Basic -and Applied Science with Bio-engineering and Synthetic Biology. We focus our attention on the structure-property relationship of the unique S-layer protein assembly from Staphylothermus marinus (S. marinus), an extremophillic archaea bacterium isolated from deep sea black smoker vents. S. marinus is a strict anaerobic sulfur reducing archaeon that utilizes peptides as a carbon source and grows at temperatures up to 98C. It has a proteinaceous S-layer consisting of elongated Tetrabrachion units arranged in a 3D-network that forms a protein-protein heterocomplex with the hyperthermostable protease, called STABLE. As a consequence of its adaptation to the extreme conditions at the deep sea volcano, the S-layer proteins of S. marinus are characterized by an unusual stability. The proposed research will focus on investigating structural and mechanistic features of (i) the 3D-assembly mechanism of Tetrabrachion to form a quasi-crystalline S-layer, (ii) the molecular mechanisms of complex formation between Tetrabrachion and STABLE and (iii) the extreme stability of S-layer components as a foundation for bio-engineering and synthetic biology. All these studies involve a combination of structural, biophysical and synthetic approaches and offer undergraduate and graduate students a great variety of different skills in modern integrated research programs. ImportanceSince their discovery, archaea have been under intense scrutiny due to their unique adaptations to the extreme environments in which they thrive. It is suggested that life has hyperthermophilic origins, and thus the study of these organisms provides insight into the biology of early life forms. In addition, understanding the molecular mechanisms of their adaptation to the extreme conditions (pH, Temperature, pressure, salinity, ionic strength) makes these microorganisms a perfect research tool for biotechnological developments. Outcome and BenefitThe expected outcome is to obtain mechanistic and chemical information about the extreme stability of archaeal protein assemblies. Understanding the molecular basis of their unique adaptation, will open new perspectives on our understanding of the origin of life and will provide new insights into development of biotechnological applications in the broad fields of monitoring, remediation and catalytic nano-devices. Over the next 5 years, it is expected that 3-4 graduate students be engaged in this research program at any given time. Our findings will guide the design and analysis of artificial S-layer assemblies with great impact on Canada's biotech sector.

工作的性质我的研究计划的长期目标是研究古细菌表面层（S层）组件的极端稳定性和独特的特性，以实现在各种生物技术领域中设计纳米devices的最终目的。该研究计划将基本科学与生物工程和合成生物学结合在一起。我们将注意力集中在葡萄球菌马林斯（S. marinus）的独特S层蛋白质组件的结构 - 质体关系上，这是一种从深海黑色吸烟者通风孔中分离出来的极端细胞古细菌。 S. marinus是一种严格的厌氧硫，可减少古老的古代，它利用肽作为碳源，并在温度下生长至98℃。它具有蛋白质S层，该蛋白质由伸长的四局部单元组成，该单元在3D网络中排列，该单元形成蛋白质 - 蛋白质杂色的杂质蛋白质杂膜，并具有过度稳定的蛋白酶，称为稳定。由于它适应了深海火山的极端条件，S. marinus的S层蛋白的特征是异常稳定性。拟议的研究将着重于研究（i）四abrachion的3D组装机制形成准晶体式S层S层，（ii）四边形和稳定与稳定和（iii）s层构成的极端稳定性的分子机制，即稳定性的生物基础，并建立了Bio-nistic stenty and-nectity and Cyntist and-inkineering and ingennicnering and-ingresnicnering。所有这些研究涉及结构，生物物理和合成方法的结合，并为本科生和研究生提供了现代综合研究计划的各种不同技能。重要的是，由于他们对极端环境的独特改编，古细菌一直受到严格的审查。有人建议生命具有超育的起源，因此对这些生物的研究提供了对早期生命形式的生物学的见解。此外，了解它们适应极端条件的分子机制（pH，温度，压力，盐度，离子强度）使这些微生物成为生物技术发展的理想研究工具。结果和受益的预期结果是获得有关古细菌组件的极端稳定性的机械和化学信息。了解其独特适应的分子基础，将对我们对生命起源的理解开辟新的看法，并将为在监测，补救和催化纳米驱动器的广泛领域提供新的见解。在接下来的5年中，预计在任何给定时间都会有3-4名研究生参加此研究计划。我们的发现将指导人工S层组件的设计和分析，对加拿大的生物技术领域产生了巨大影响。