OFI SF7 Assessing the effects of Ocean Alkalinity Enhancement (OAE) on the marine microbiome

OFI SF7 评估海洋碱度增强 (OAE) 对海洋微生物组的影响

• 批准号: 576785-2022
• 负责人: LaRoche, JulieJ
• 金额: $ 2.11万
• 依托单位: Dalhousie University
• 依托单位国家: 加拿大
• 项目类别: Alliance Grants
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=745327
• 关键词: OFI; SF7; Assessing; effects; Ocean

Ocean alkalinity enhancement (OAE) is rapidly gaining support within the proposed ocean-basedcarbon dioxide removal (ocean-CDR) approaches. Theoretically, OAE is a CDR approach that should have minimal adverse impact on the marine ecosystem, while having the potential torestore the detrimental effects of anthropogenic CO2-enhanced ocean acidification in impacted regions of the ocean (e.g. coral reefs). However, there is a lack of experimental datadocumenting the OAE effect on marine life and an urgent need to obtain this data for the marine microbiome (phytoplankton, bacteria and archaea) given their position at the base of the foodchain. Planetary Technologies is developing OAE-based CDR and is planning to use BedfordBasin as a pilot study site. The applicant proposes to conduct bioassay experiments to assess the potential effects of OAE on the microbiome. The bioassay experiments consist of short-term (up to 72 hours) bottle-incubations with natural microbial communities from the Bedford Basin with (treatment) and without (control) the addition of the alkaline substrate magnesium hydroxide,during four seasons over a 12-month period. High-throughput sequencing of nucleic acids (DNA and RNA) extracted from the microbiome of the control and treatment incubations will becompared to each other and to the initial community obtained from ongoing time-seriessampling. The experimental results and database will be shared with Planetary and othershareholders to establish a baseline for environmental assessment and guide the initial trials oftheir approach to minimize the potential unforeseen effects of OAE on the living ecosystem.

海洋碱度增强（OAE）在拟议的基于海洋的二氧化碳去除（ocean-CDR）方法中迅速获得支持。从理论上讲，OAE 是一种 CDR 方法，应该对海洋生态系统产生最小的不利影响，同时有可能恢复受影响海洋区域（例如珊瑚礁）中人为二氧化碳增强海洋酸化的不利影响。然而，缺乏记录 OAE 对海洋生物影响的实验数据，鉴于海洋微生物组（浮游植物、细菌和古细菌）位于食物链的底部，迫切需要获得这些数据。 Planetary Technologies 正在开发基于 OAE 的 CDR，并计划使用 BedfordBasin 作为试点研究地点。申请人建议进行生物测定实验来评估OAE对微生物组的潜在影响。生物测定实验包括对来自贝德福德盆地的天然微生物群落进行短期（最多 72 小时）瓶内培养，在 12 个季节的四个季节中添加（处理）和不添加（对照）碱性底物氢氧化镁。月期间。从对照和治疗培养物的微生物组中提取的核酸（DNA 和 RNA）的高通量测序将相互比较，并与从持续的时间序列采样中获得的初始群落进行比较。 实验结果和数据库将与行星公司和其他股东共享，以建立环境评估基线并指导其方法的初步试验，以尽量减少 OAE 对生物生态系统潜在的不可预见的影响。