Biomolecular Infrastructure for Detection of Radioisotopes, Fluorescence, Chemiluminescence

用于检测放射性同位素、荧光、化学发光的生物分子基础设施

• 批准号: RTI-2023-00416
• 负责人: Bayfield, Mark
• 金额: $ 10.16万
• 依托单位: York University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=745609
• 关键词: Biomolecular; Infrastructure; Detection; Radioisotopes; Fluorescence

A Typhoon NIR Biomolecular Imager with a Blue Laser and an Azure 280 Gel Imager are requested by Discovery Grant funded applicants for the purpose of supporting multiple research programs in the Life Science Building (LSB) at York University. York University's Life Science Building supports 26 primary investigators, including at least 15 in the Departments of Biology and Chemistry that are supported by NSERC Discovery Grants. These research programs rely heavily on the ability to detect and quantitate levels of radioisotopes, fluorophores and chemiluminescence (ECL) linked to or associated with nucleic acids and proteins of interest. The only instrument for radioisotope detection on gels and membranes (i.e. 32P labelled RNA and DNA, or 35S labelled proteins) in the LSB was a Typhoon Biomolecular Imager purchased in 2008. This imager was also the only instrument in the LSB that was capable of detecting blue wavelength fluorescently labelled proteins or nucleic acids. Recently, the Typhoon imager purchased in 2008 broke down, and the manufacturer has indicated that it is no longer supported and cannot be repaired. This means that it is currently impossible to perform quantitative gel or membrane-based radioisotope or fluorescence detection in the LSB. ECL is currently detected using a MicroChemi 2.0 that is at the end of its lifespan, as it is now routinely giving errors and for which parts or support is no longer provided by the manufacturer. Thus the need for a new set of instruments that can be used for radioisotope, fluorescence and ECL detection is extremely urgent, as research programs that rely on this functionality have had their research productivity severely hampered. There is a wide breadth of Discovery grant supported groups in the LSB studying diverse research topics including but not limited to RNA processing, epigenetics, signal transduction, post-translational modification of proteins by ubiquitin and SUMO, host-microbe interactions, cellular differentiation and cellular adaptations to stress. The requested instruments will represent critical infrastructure supporting all of these programs. The instruments will be used by graduate students and other highly qualified personnel, gaining a valuable training for future careers in academia or industry. The application will benefit applicants and trainees from a diverse breadth of equity seeking groups.

Discovery Grant资助的申请人要求使用带有蓝色激光器和Azure 280凝胶成像仪的台风NIR生物分子成像仪，目的是支持约克大学生命科学大楼（LSB）的多个研究计划。约克大学的生命科学大楼支持26名主要研究人员，其中包括NSERC Discovery Grants支持的生物学和化学部门至少有15个。这些研究计划在很大程度上依赖于检测和定量与核酸和感兴趣的蛋白质相关或相关的放射性病，荧光团和化学发光（ECL）水平的能力。 LSB中唯一在凝胶和膜（即32p标记的RNA和DNA或35S标记蛋白的标签标记的RNA和DNA）上检测的唯一的仪器是2008年购买的台风生物分子的想象器。该成像器也是LSB中唯一能够检测的仪器蓝色波长荧光标记为蛋白质或核酸。最近，2008年购买的台风成像师崩溃了，制造商表示不再支持它，无法修复。这意味着目前无法在LSB中执行定量凝胶或基于膜的放射性同位素或荧光检测。目前使用Microchemi 2.0检测到ECL，该微化学2.0在其寿命末期，因为现在通常会出现错误，并且制造商不再为哪些零件或支持提供。因此，需要用于放射性同位素，荧光和ECL检测的新工具的需求极为紧迫，因为依靠该功能的研究计划严重阻碍了他们的研究生产力。 LSB中有广泛的发现赠款支持各种研究主题，包括但不限于RNA处理，表观遗传学，信号转导，泛素和Sumo对蛋白质的翻译后修饰，宿主宿主相互作用，细胞分化和细胞分化以及细胞分化以及细胞分化和细胞差异适应压力。请求的工具将代表支持所有这些程序的关键基础架构。这些工具将由研究生和其他高素质的人员使用，并为学术界或行业的未来职业提供宝贵的培训。该申请将使来自各种股权寻求股票集团的各种广度的申请人和受训者受益。