Evolution of mosquito-virus interactions: range expansions, viral coinfections and mosquito behaviour

蚊子-病毒相互作用的演变：范围扩大、病毒共感染和蚊子行为

• 批准号: RGPIN-2020-05200
• 负责人: Hunter, Fiona
• 金额: $ 2.91万
• 依托单位: Brock University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=744761
• 关键词: Evolution; mosquito; virus; interactions; range

There are three research foci in my lab. (1) TAXONOMY and SYSTEMATICS  We will be studying the incursions of two mosquito species into Canada, namely Aedes albopictus (Asian Tiger mosquito) and Aedes aegypti (Yellow Fever mosquito), by tracing the phylogenetic relationships of established and novel populations using DNA sequences. We will also undertake a revision of 8 species in the mosquito genus Culiseta to determine how they are related to one another; such information is necessary for understanding the transmission of Eastern Equine Encephalitis virus. (2) BEHAVIOURAL ECOLOGY  Earlier work on the effects of sugar-feeding among the blood-feeding Diptera has suggested that honeydew sugar meals offer enhanced flight capabilities and may protect mosquitoes from some protozoan infections. In contrast, nectar meals may offer enhanced vitellogenesis which presumably leads to increased egg production. We aim to examine the effects of honeydew vs. nectar meals on longevity and fecundity in mosquitoes. This will be followed by choice experiments in which we determine whether the gender, age, size, mating status and/or infection status of mosquitoes influences the type of sugar-meal ingested. (3) DISEASE TRANSMISSION In addition to ongoing vector competence studies to determine which mosquitoes are able to transmit which viruses, we are interested in the effects of dual infection with two strains of the same virus as well as with two different viruses. We intend to study Zika virus, Mayaro virus and eventually Dengue virus and Yellow Fever virus. Co-infection and superinfection studies will be done in cell culture first and then in live, infected mosquitoes in Brock's Containment Level 3 Lab with Insectary.

我的实验室中有三个研究重点。 （1）分类学和系统学，我们将通过追踪使用DNA序列的系统生理关系和新型种群来研究两种蚊子侵入加拿大的入侵加拿大，即白optictus（亚洲老虎蚊子）和伊蚊（亚洲老虎蚊子）（黄热蚊子）。我们还将在蚊子属中对8种物种进行修订，以确定它们之间的关系。这种信息对于理解东马脑炎病毒的传播是必要的。 （2）行为生态学早些时候对喂食双翅目中糖进粉的影响的作用提出，蜜糖糖粉具有增强的飞行能力，并可能保护蚊子免受某些原生动物的感染。相比之下，花蜜餐可能会提供增强的叶片发生，这大概会导致卵产量增加。我们的目的是研究蜜糖与花蜜餐对蚊子长寿和繁殖力的影响。随后将进行选择实验，在这些实验中，我们确定蚊子的性别，年龄，大小，交配状态和/或感染状态是否会影响摄入的糖粉的类型。 （3）疾病的传播除了进行持续的媒介能力研究以确定哪些蚊子能够传播哪些病毒，我们对两种相同病毒菌株的双重感染以及两种不同病毒感兴趣。我们打算研究寨卡病毒，玛雅罗病毒，并最终研究登革热病毒和黄热病病毒。共同感染和超级感染研究将首先在细胞培养中进行，然后在Brock的“遏制3级实验室”中现场感染的蚊子中进行。