Identifying the systemic mode of action of microbial solutions controlling foodborne pathogen E. coli O157 shedding

确定控制食源性病原体大肠杆菌 O157 脱落的微生物解决方案的系统作用模式

• 批准号: 561309-2020
• 负责人: Guan, LeluoL
• 金额: $ 10.45万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Alliance Grants
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=743318
• 关键词: Identifying; systemic; mode; action; microbial

Food safety and one health are the key challenges faced by the Canadian beef industry, especially in the transmission of zoonotic infectious diseases from farm animals, the farm environment or food to humans. In the last two decades numerous large outbreaks of Shiga toxin-producing Escherichia coli O157:H7 (STEC O157) have led to tens of millions of dollars in losses to the beef industry. Although it has been a few years since the XL foods outbreak, another incident is inevitable and there is a need for the Canadian beef industry to continue to pursue new methods to reduce the incidence of this bacterium in the food production chain. Cattle are the main reservoir for STEC O157, and the transmission of this pathogen occurs through fecal shedding into the environment which then contaminates vegetable crops, hides and meat during processing. Several preharvest interventions had been tested, including vaccines, and feed additives, but none of the methods can effectively control fecal shedding of E. coli O157:H7 in cattle. Different from previous approaches, current proposed NSERC Alliance project aims to develop a novel direct fed microbe-based approach to regulate host-microbial interactions for better gut health and eliminate or lower STEC colonization in the gut of cattle. The information obtained and technologies developed by the project will provide industrial applicable solutions to enhance cattle health and reduce the prevalence of this pathogen in the food chain. The overall knowledge generated from this project will help industry to develop and implement cost-effective technologies to improve food safety along the beef supply chain that will benefit agricultural stakeholders and other Canadian stakeholders.

食品安全和一种健康是加拿大牛肉行业面临的主要挑战，尤其是在农场动物，农场环境或食物的人畜传染病传播中。在过去的二十年中，大量爆发了志贺毒素的大肠杆菌O157：H7（STEC O157）导致牛肉行业造成了数千万美元的损失。尽管自XL食品爆发以来已经有几年了，但另一个事件是不可避免的，并且需要加拿大牛肉行业继续采用新方法来减少食品生产链中这种细菌的发生率。牛是STEC O157的主要储层，这种病原体的传播是通过粪便脱落到环境中的，然后在加工过程中污染了蔬菜，生育和肉类。已经测试了几种预览前干预措施，包括疫苗和饲料添加剂，但是这些方法都无法有效地控制牛大肠杆菌O157：H7的粪便脱落。与以前的方法不同，当前提出的NSERC联盟项目旨在开发一种新型的基于喂养微生物的直接方法，以调节宿主微生物相互作用，以改善肠道健康，并消除牛肠道中的STEC殖民化。该项目获得的信息和技术将提供工业适用的解决方案，以增强牛健康并降低食物链中这种病原体的普遍性。该项目产生的总体知识将有助于行业开发和实施具有成本效益的技术，以改善将使农业利益相关者和其他加拿大利益相关者受益的牛肉供应链的食品安全。