Identifying the systemic mode of action of microbial solutions controlling foodborne pathogen E. coli O157 shedding

确定控制食源性病原体大肠杆菌 O157 脱落的微生物解决方案的系统作用模式

• 批准号: 561309-2020
• 负责人: Guan, LeluoL
• 金额: $ 10.45万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Alliance Grants
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=743318
• 关键词: Identifying; systemic; mode; action; microbial

Food safety and one health are the key challenges faced by the Canadian beef industry, especially in the transmission of zoonotic infectious diseases from farm animals, the farm environment or food to humans. In the last two decades numerous large outbreaks of Shiga toxin-producing Escherichia coli O157:H7 (STEC O157) have led to tens of millions of dollars in losses to the beef industry. Although it has been a few years since the XL foods outbreak, another incident is inevitable and there is a need for the Canadian beef industry to continue to pursue new methods to reduce the incidence of this bacterium in the food production chain. Cattle are the main reservoir for STEC O157, and the transmission of this pathogen occurs through fecal shedding into the environment which then contaminates vegetable crops, hides and meat during processing. Several preharvest interventions had been tested, including vaccines, and feed additives, but none of the methods can effectively control fecal shedding of E. coli O157:H7 in cattle. Different from previous approaches, current proposed NSERC Alliance project aims to develop a novel direct fed microbe-based approach to regulate host-microbial interactions for better gut health and eliminate or lower STEC colonization in the gut of cattle. The information obtained and technologies developed by the project will provide industrial applicable solutions to enhance cattle health and reduce the prevalence of this pathogen in the food chain. The overall knowledge generated from this project will help industry to develop and implement cost-effective technologies to improve food safety along the beef supply chain that will benefit agricultural stakeholders and other Canadian stakeholders.

食品安全和健康是加拿大牛肉行业面临的主要挑战，特别是在农场动物、农场环境或食品向人类传播人畜共患传染病方面。在过去的二十年中，产志贺毒素大肠杆菌 O157:H7 (STEC O157) 的多次大规模爆发给牛肉行业造成了数千万美元的损失。尽管XL Foods爆发已经过去了几年，但再次发生事件是不可避免的，加拿大牛肉行业需要继续寻求新方法来减少这种细菌在食品生产链中的发病率。牛是 STEC O157 的主要宿主，这种病原体的传播是通过排泄物排泄到环境中，然后在加工过程中污染蔬菜作物、皮革和肉类。已经测试了几种收获前干预措施，包括疫苗和饲料添加剂，但没有一种方法可以有效控制牛粪便中大肠杆菌 O157:H7 的排出。与以前的方法不同，当前提出的 NSERC 联盟项目旨在开发一种基于直接饲喂微生物的新型方法来调节宿主-微生物相互作用，以改善肠道健康并消除或降低牛肠道中的 STEC 定植。该项目获得的信息和开发的技术将提供工业适用的解决方案，以增强牛的健康并减少这种病原体在食物链中的流行。该项目产生的整体知识将帮助行业开发和实施具有成本效益的技术，以改善牛肉供应链的食品安全，这将使农业利益相关者和其他加拿大利益相关者受益。