Advanced time-resolved optical sensing and imaging systems for biomedical and environmental applications

适用于生物医学和环境应用的先进时间分辨光学传感和成像系统

• 批准号: RGPIN-2019-07127
• 负责人: Fang, Qiyin
• 金额: $ 3.35万
• 依托单位: McMaster University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2021
• 资助国家: 加拿大
• 起止时间: 2021-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=739322
• 关键词: Advanced; time; resolved; optical; sensing

Recent advances in photonics and electronics technologies are one of the driving forces behind many novel photonics devices development. Among these device technologies, optical sensing and imaging have been investigated for applications in life sciences research (e.g. microscopic imaging and analytical instruments), environmental sensing (e.g. water quality monitoring) and medical diagnosis (e.g. endoscopic imaging and laboratory test instruments). The majority of the instruments and devices operate by measuring the amplitude of the light intensity in the spatial (e.g. imaging) and spectral (e.g. spectroscopy) domain. Time-dependent intensity variation, especially at high speed, are not as widely used, even when many feasibility studies demonstrated that they provide important information for many applications. For example, comparing to intensive based measurements, fluorescence lifetime imaging (FLIM) can provide more efficient measure of protein-protein interaction at 1-10 nm scale, which could be very useful in drug discovery process. However, there is no commercial high content screening instrument with a high resolution FLIM capability for measuring protein-protein interaction. Another example is dissolved oxygen (DO) measurements. Although commercial frequency modulated DO instruments exist for more than 10 years, miniaturized lab-on-chip DO measurements are still relying on steady-state techniques and usually cost over $1000 each. In the proposed research program, we plan to study the integration of photonics, microelectronics, and mechanical components into next generation time-resolved optical sensors/imagers. Specifically, we will 1)develop technologies towards a FLIM microscope with the highest acquisition speed with high spatial and temporal resolution; 2)develop integrated time-resolved compact optofluidics sensor devices capable of continuous measurements while remain low-cost; 3)develop customized computational algorithms tailored to the detection technologies for accurate and fast time-domain parameter retrieval. The proposed advanced sensing and imaging technology program will be supported by the world class Micro/Nano material synthetization, component fabrication, characterization, and device integration facilities available at McMaster. The program will focus on integrated time-domain optical sensing and imaging device technology development. The applications of such technologies include life science analytical instruments, automated microscopy for drug discovery, and environmental monitoring. Research in these applications will be supported by project-based funding mechanisms including NSERC, OCE, and CIHR.

光子学和电子技术的最新进展是许多新型光子器件开发背后的驱动力之一。在这些设备技术中，光学传感和成像已被研究用于生命科学研究（例如显微成像和分析仪器）、环境传感（例如水质监测）和医疗诊断（例如内窥镜成像和实验室测试仪器）。 大多数仪器和设备通过测量空间（例如成像）和光谱（例如光谱）域中的光强度幅度来运行。与时间相关的强度变化，尤其是在高速情况下，并未得到广泛使用，即使许多可行性研究表明它们为许多应用提供了重要信息。例如，与基于密集的测量相比，荧光寿命成像 (FLIM) 可以在 1-10 nm 尺度上提供更有效的蛋白质-蛋白质相互作用测量，这在药物发现过程中非常有用。然而，目前还没有具有高分辨率 FLIM 功能的商用高内涵筛选仪器用于测量蛋白质-蛋白质相互作用。另一个例子是溶解氧 (DO) 测量。尽管商用调频 DO 仪器已存在 10 多年，但小型片上实验室 DO 测量仍然依赖于稳态技术，并且每台仪器的成本通常超过 1000 美元。 在拟议的研究计划中，我们计划研究将光子学、微电子学和机械部件集成到下一代时间分辨光学传感器/成像仪中。具体来说，我们将1）开发具有最高采集速度和高空间和时间分辨率的FLIM显微镜技术； 2）开发集成的时间分辨紧凑型光流控传感器装置，能够连续测量，同时保持低成本； 3）开发适合检测技术的定制计算算法，以实现准确、快速的时域参数检索。 拟议的先进传感和成像技术计划将得到麦克马斯特大学世界一流的微/纳米材料合成、元件制造、表征和设备集成设施的支持。该计划将重点关注集成时域光学传感和成像器件技术的开发。此类技术的应用包括生命科学分析仪器、用于药物发现的自动显微镜和环境监测。这些应用的研究将得到基于项目的资助机制的支持，包括 NSERC、OCE 和 CIHR。