Detection of Legionella pneumophila using aptamer-coated nanoparticles.

使用适配体包被的纳米颗粒检测嗜肺军团菌。

基本信息

批准号：
564718-2021
负责人：
Faucher, Sébastien
金额：
$ 1.46万
依托单位：
McGill University
依托单位国家：
加拿大
项目类别：
Alliance Grants
财政年份：
2021
资助国家：
加拿大
起止时间：
2021-01-01 至 2022-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=722166
关键词：
Detection Legionella pneumophila using aptamer

项目摘要

Legionella pneumophila is the causative agent of Legionnaires' disease, an acute pulmonary infection in humans. Several large outbreaks of LD occur every year, each one affecting hundreds of people and resulting in the death of about 10% of the affected population. For example, the Quebec City outbreak in 2012 resulted in 183 cases and 13 deaths. L. pneumophila grows in water systems and is transmitted to human by inhalation of aerosols. To prevent outbreak of Legionnaires' disease it is imperative to manage water systems adequately and take appropriate action when L. pneumophila is detected in them. The current available detection methods are time consuming and require expert technical skills. We are proposing to develop a novel detection method based on nanoparticles coated with L. pneumophila-specific recognition molecules called aptamers. To do so, we will evaluate the performance of this method with different strains of L. pneumophila and determine its cross reactivity with other microbes typically found in water systems. Finally, we will adapt this approach with an existing portable colorimetric detection platform. This project will develop a new portable, easy to use, and cost-effective method for routine monitoring of water systems that will help reduce the risk and burden of Legionnaires' disease.

嗜肺军团菌是军团病（一种人类急性肺部感染）的病原体。每年都会发生数次大规模的LD暴发，每次都会影响数百人，并导致约10%的受影响人口死亡。例如，2012年魁北克市爆发的疫情导致183例病例和13人死亡。嗜肺军团菌生长在水系统中，通过吸入气溶胶传播给人类。为了防止军团病的爆发，必须对水系统进行充分管理，并在其中检测到嗜肺军团菌时采取适当的行动。目前可用的检测方法非常耗时并且需要专业的技术技能。我们提议开发一种基于纳米颗粒的新型检测方法，该纳米颗粒涂有嗜肺军团菌特异性识别分子（称为适体）。为此，我们将评估该方法在不同嗜肺军团菌菌株上的性能，并确定其与水系统中常见的其他微生物的交叉反应性。最后，我们将利用现有的便携式比色检测平台来调整这种方法。该项目将开发一种新的便携式、易于使用且具有成本效益的方法，用于水系统的日常监测，这将有助于降低退伍军人病的风险和负担。