CoastCheck: Establishment of robust eDNA assays to assess organic pollution impacts on infaunal macrobenthic invertebrate communities in coastal waters of British Columbia

CoastCheck：建立强大的 eDNA 检测方法，评估有机污染对不列颠哥伦比亚省沿海水域动物群大型底栖无脊椎动物群落的影响

• 批准号: 531846-2018
• 负责人: Helbing, Caren
• 金额: $ 4.84万
• 依托单位: University of Victoria
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2021
• 资助国家: 加拿大
• 起止时间: 2021-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=719712
• 关键词: CoastCheck; Establishment; robust; eDNA; assays

Provincially- and federally-mandated programs for wastewater and environmental effects monitoring require an assessment of the health of marine invertebrate communities that live in the soft sediments at the sea bottom. Biologica has provided taxonomic identification services for government and industry for over 20 years using traditional sorting and visual classification methods. However, this approach is very time-consuming, costly, and technically challenging. There is a need to expand and improve upon the reliability of monitoring these invertebrates. All living organisms are defined by their genetic material and this DNA is released into the environment. This "environmental DNA (eDNA)" can be detected using sensitive and accurate molecular tests on water and sediment samples. The overall project goal is to develop robust, targeted eDNA-based tests to assess the impact of organic pollution on invertebrates that live in sediments of marine areas. The CRD project combines the expertise of academic (University of Victoria), industry (Biologica), and wastewater management service providers (the Capital Regional District of Victoria and the Greater Vancouver Sewerage and Drainage District) to address two main objectives: 1) to develop and validate eDNA tests for verified specimens of invertebrate species of interest, and 2) to create a robust, standardized eDNA field sampling approach for marine coastal community assessments of organic pollution impacts using invertebrates that live in sea bottom sediments. This will be accomplished in three specific aims: 1) to obtain high quality DNA sequences from verified specimens, 2) to develop and validate eDNA tests for select invertebrates, and 3) to apply the eDNA tests in real-world scenarios. The routine application of these new eDNA tools will revolutionize the taxonomy service industry and how environmental impacts of wastewater are assessed.

在废水和环境效果监测的省份和联邦政府的计划中，需要评估生活在海底软沉积物中的海洋无脊椎动物群落的健康状况。 Biologica已使用传统的分类和视觉分类方法为政府和行业提供了分类学识别服务。但是，这种方法非常耗时，昂贵且在技术上具有挑战性。需要扩展和提高监测这些无脊椎动物的可靠性。 所有生物体都由其遗传物质定义，并且该DNA被释放到环境中。可以使用在水和沉积物样品上进行敏感和准确的分子测试来检测此“环境DNA（EDNA）”。 总体项目目标是开发有针对性的基于EDNA的测试，以评估有机污染对生活在海洋地区沉积物中的无脊椎动物的影响。 CRD项目结合了学术（维多利亚大学），工业（Biologica）和废水管理服务提供商（维多利亚州的资本区域和更大的温哥华下水道和排水区）的专业知识，以解决两个主要目标：1），以开发和验证EDNA测试，以验证EDNA测试，以验证一种标准的标准范围，以实现范围的范围，以下标准的范围，并采用了Marrine and Narte of Inverte and Same and same and same and same and same and 2）社区对有机污染的评估会影响生活在海底沉积物中的无脊椎动物。这将在三个具体目标中完成：1）从经过验证的样品中获得高质量的DNA序列，2）开发和验证选择无脊椎动物的EDNA测试，而3）在实际情况下应用EDNA测试。 这些新的EDNA工具的常规应用将彻底改变分类服务行业，以及如何评估废水的环境影响。