Regulation of transcriptional responses to intercellular signaling during development

发育过程中细胞间信号传导转录反应的调节

• 批准号: RGPIN-2020-06100
• 负责人: Nilson, Laura
• 金额: $ 2.62万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=717737
• 关键词: Regulation; transcriptional; responses; intercellular; signaling

The broad goal of my research program is to understand how cells become specialized as an animal develops. This specialization process involves mechanisms that turn some genes on turn others off, and ultimately this profile of gene activity leads each cell to adopt the shape, function, and/or behaviour that allows it to fulfill its function within the tissue. Most tissues are made up of a number of different types of cells, and these multiple cell types are often organized into very specific spatial patterns. Such a pattern reflects the ability of each individual cell to detect where it lies within the tissue and regulate its genes appropriately. The work in my lab is focused on understanding how each cell in the developing tissue senses its position, receives biochemical instructions to guide its specialization, and turns on and off the correct set of genes for its position and future function. By studying the developing fruit fly ovary as a model tissue, we recently discovered that complex and specific patterns of gene activity can be generated by biochemical signals that are present in different regions of the tissue. Where they overlap, they work together to turn genes on and off. We now propose to further study how these signals collaborate, as well as the details of how they choose which genes to activate and which to inhibit. The genes of the fruit fly are actually very similar to those of humans, and over 75% of fruit fly genes have a recognizable human equivalent. This similarity suggests that the genetic lessons that we learn by studying the fruit fly will tell us a lot about how genes are regulated in humans and other animals.

我的研究计划的总体目标是了解细胞如何随着动物的发育而变得特化。这种专门化过程涉及打开某些基因、关闭其他基因的机制，最终，这种基因活性特征导致每个细胞采用允许其在组织内履行其功能的形状、功能和/或行为。大多数组织由许多不同类型的细胞组成，并且这些多种细胞类型通常被组织成非常特定的空间模式。这种模式反映了每个单独的细胞检测其在组织内的位置并适当调节其基因的能力。 我实验室的工作重点是了解发育组织中的每个细胞如何感知其位置，接收生化指令以指导其专业化，并根据其位置和未来功能打开和关闭正确的基因组。通过研究发育中的果蝇卵巢作为模型组织，我们最近发现组织不同区域中存在的生化信号可以产生复杂且特定的基因活性模式。在它们重叠的地方，它们共同作用来打开和关闭基因。我们现在建议进一步研究这些信号如何协作，以及它们如何选择激活哪些基因和抑制哪些基因的细节。 果蝇的基因实际上与人类的基因非常相似，超过 75% 的果蝇基因具有可识别的人类基因。这种相似性表明，我们通过研究果蝇学到的遗传教训将告诉我们很多关于人类和其他动物的基因是如何调节的。