Nanoparticle Tracking Analysis Platform for in vitro Characterization of Engineered and Natural Nanoparticles in Biological Fluids

用于生物流体中工程纳米颗粒和天然纳米颗粒体外表征的纳米颗粒跟踪分析平台

• 批准号: RTI-2021-00374
• 负责人: Labouta, Hagar
• 金额: $ 10.58万
• 依托单位: University of Manitoba
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=718584
• 关键词: Nanoparticle; Tracking; Analysis; Platform; vitro

Nanoparticles (NPs) are particles in the nanometer range (1/1000th of a millimeter) with size-dependent properties. Recent research in material science has resulted in successful engineering of wide varieties of NPs with different composition and properties (e.g. size). There are also NPs that are naturally produced by the body and are responsible for important biological functions. Extracellular vesicles (EVs) are natural NPs secreted from the cells to communicate with other cells. Despite years of excellent individual investigations, our ability to predict the behaviour of NPs in the biological environment is still limited. This is because research approaches rely on relating the behavior of NPs to their native properties in simple artificial solutions. However, the properties of NPs change when they interact with the different biological fluids present in the body. In some situations, NPs could agglomerate and become unstable in biological fluids. The concentration of NPs is expected to directly impact their biological performance. It is crucial for material scientists, nanoscientists and EV biologists at the University of Manitoba to access novel technologies that allow for unambiguous determination of size and concentration of NPs in biological fluids. This will allow researchers at UManitoba to generate new knowledge on the true behaviour of natural and engineered NPs, allowing for competition in this area on the global stage and ultimately enabling engineers and scientists to develop NPs with predictable and efficient behaviour in the body. The requested nanoparticle tracking analysis (NTA) platform will fill this important void at UManitoba, and will help establish UManitoba as a leading international centre for advanced nanomaterial and EV biology. The NTA platform enables real-time single particle tracking using the light scattering of NPs while they are diffusing in fluids. This is the only technique that can generate reliable, precise and accurate size and concentration measurements of NPs in complex biological fluids. Fluorescence detection capability of the newest NTA platforms can detect sub-populations within a mixture of NPs that differ in composition. NTA has become the gold standard for these measurements by regulatory agencies. Several researchers at UManitoba will use this tool to support fundamental research programs to study the interaction of engineered NPs and EVs with biomolecules and cells, and explore new opportunities for developing new drug delivery systems with predictable behaviour in the body. Our HQP will be provided with unique opportunities to engage in cutting-edge training required for careers in material science, material and biomedical engineering, nanotechnology and biology. The purchase of this NTA equipment is thus timely to stimulate advances in the areas of nanoscience and EV biology, and train new personnel in state-of-the-art nanotechnology skills that will satisfy Canadian market needs.

纳米颗粒（NP）是具有尺寸依赖性特性的纳米范围（毫米1/1000）的颗粒。材料科学的最新研究导致了具有不同组成和特性（例如尺寸）的广泛的NP的成功工程。也有人体自然产生的NP，并负责重要的生物学功能。细胞外囊泡（EV）是从细胞中分泌的天然NP，与其他细胞进行通信。 尽管经过多年的个人调查，但我们预测NP在生物环境中的行为的能力仍然有限。这是因为研究方法依赖于将NP与其本地特性相关联在简单的人工解决方案中。但是，NPS与体内存在的不同生物流体相互作用时，它们的特性会发生变化。在某些情况下，NP可以在生物流体中凝结并变得不稳定。 NP的浓度有望直接影响其生物学性能。对于曼尼托巴大学的物质科学家，纳米科学家和EV生物学家来说，这对于访问新型技术至关重要，可以明确确定生物体流体中NP的大小和浓度。这将使Umanitoba的研究人员能够为自然和工程NP的真实行为产生新的知识，从而使该领域的全球舞台上的竞争，并最终使工程师和科学家能够开发具有人体可预测和高效行为的NP。 要求的纳米颗粒跟踪分析（NTA）平台将在Umanitoba填补这一重要空隙，并将帮助建立Umanitoba，成为高级纳米材料和EV生物学的领先国际中心。 NTA平台可以使用NP的光散射在流体中扩散。这是唯一可以在复杂的生物流体中生成可靠，精确和准确的大小和浓度测量的技术。最新的NTA平台的荧光检测能力可以检测组成不同的NP的混合物中的子选集。 NTA已成为监管机构这些测量的黄金标准。 Umanitoba的一些研究人员将使用此工具来支持基础研究计划，以研究工程NPS和EV与生物分子和细胞的相互作用，并探索开发具有可预测行为的新药物输送系统的新机会。我们的HQP将获得独特的机会，以进行材料科学，材料和生物医学工程，纳米技术和生物学的职业所需的尖端培训。因此，购买该NTA设备的购买是及时刺激纳米科学和电动汽车生物学领域的进步，并以最先进的纳米技术技能培训新人员，这些技能将满足加拿大的市场需求。