Mechanistic insights into the temperature regulation of plant immunity

植物免疫温度调节的机制见解

• 批准号: RGPIN-2020-04117
• 负责人: Castroverde, ChristianDanve
• 金额: $ 2.04万
• 依托单位: Wilfrid Laurier University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=716311
• 关键词: Mechanistic; insights; into; temperature; regulation

Plant diseases combined with climate change-associated temperature anomalies pose serious threats to agriculture and food security. Previous studies have led to significant insights into how plants defend themselves against pathogens and pests. One of these crucial defence responses is the production of the hormone salicylic acid (SA), which allows host plants to globally control thousands of genes necessary to resist a broad range of pathogens. However, the molecular mechanisms underlying how environmental factors (e.g. elevated temperature) affect the plant SA pathway are not well-understood. My research aims to address this important knowledge gap. My previous work showed that elevated temperature suppresses SA production in the model plant Arabidopsis thaliana by downregulating the expression of CALMODULIN-BINDING PROTEIN 60g (CBP60g). CBP60g encodes a master transcription factor controlling many immune regulators in plants, including genes involved in producing SA. Because the CBP60g gene is a central hub in integrating temperature and immune pathways, how it is regulated at the molecular level will provide clues on how temperature influences the plant SA pathway. Additionally, whether temperature-sensitive immunity is conserved in other plant species is also unclear. My long-term vision is to create an integrative model that defines how plants resist pathogens in the context of a changing environment. My research program will integrate molecular, genetic and biochemical approaches in order to pursue three major aims in the next five years. First, we will identify essential transcription factors of CBP60g gene expression using both in silico and in vivo methods. Second, we will perform a genetic screen to discover new regulators of CBP60g gene expression at high temperature and validate the results using mutational approaches. Third, we will determine if temperature suppression of host immunity is conserved in other plant species, by studying CBP60g gene expression and SA production of the agriculturally important tomato crop. Overall, my research program would more accurately address the complexity of plant diseases in the natural environment, by identifying the essential genes and mechanisms that lead to compromised SA production at high temperature. This knowledge should help us better understand plant resilience to various stresses and contribute to strategies in improving crop disease resistance in Canada and worldwide. For the proposed research, HQP will acquire excellent laboratory and practical skills that are important for their chosen careers.

植物病害与气候变化相关的温度异常相结合，对农业和粮食安全构成严重威胁。先前的研究对植物如何防御病原体和害虫产生了重要的见解。这些关键的防御反应之一是激素水杨酸 (SA) 的产生，它使宿主植物能够全面控制抵抗多种病原体所需的数千个基因。然而，环境因素（例如升高的温度）如何影响植物 SA 途径的分子机制尚不清楚。我的研究旨在解决这一重要的知识差距。 我之前的工作表明，升高温度通过下调钙调蛋白结合蛋白 60g (CBP60g) 的表达来抑制模式植物拟南芥中 SA 的产生。 CBP60g 编码一个主转录因子，控制植物中的许多免疫调节因子，包括参与产生 SA 的基因。由于 CBP60g 基因是整合温度和免疫途径的中心枢纽，因此如何在分子水平上对其进行调控将为温度如何影响植物 SA 途径提供线索。此外，温度敏感性免疫力在其他植物物种中是否保守也不清楚。 我的长期愿景是创建一个综合模型，定义植物如何在不断变化的环境中抵抗病原体。我的研究计划将整合分子、遗传和生化方法，以实现未来五年的三个主要目标。首先，我们将使用计算机和体内方法鉴定 CBP60g 基因表达的必需转录因子。其次，我们将进行遗传筛选，以发现高温下 CBP60g 基因表达的新调节因子，并使用突变方法验证结果。第三，我们将通过研究农业上重要的番茄作物的 CBP60g 基因表达和 SA 生产来确定宿主免疫的温度抑制是否在其他植物物种中保守。 总体而言，我的研究计划将通过识别导致高温下 SA 生产受损的基本基因和机制，更准确地解决自然环境中植物病害的复杂性。这些知识应该有助于我们更好地了解植物对各种胁迫的适应能力，并有助于制定提高加拿大和全世界作物抗病性的策略。对于拟议的研究，HQP 将获得对其所选职业非常重要的出色实验室和实践技能。