Characterizing functionalized electrode surfaces with unique spectroelectrochemical methods for biosensor development

使用独特的光谱电化学方法表征功能化电极表面，用于生物传感器的开发

• 批准号: RGPIN-2016-05528
• 负责人: Bizzotto, Dan
• 金额: $ 3.35万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=709770
• 关键词: Characterizing; functionalized; electrode; surfaces; unique

Electrochemical processes form the basis of many new technologies (eg. batteries, fuel cells, solar cells) as well as approaches to bioanalytical sensing. The portability and small footprint of these sensors Electrochemical based biosensors rely on the modification of the electrode surface with biologically active species (eg lipids, nucleic acids or enzymes) that can specifically interact with compounds of interest in the sample, and then transduce this interaction into a signal that can be measured accurately. This requires a high level of control over the preparation of the sensor surface. A biosensor must have a sufficient number of probes on the surface which are accessible by the targets in solution so that when specifically interacting, will create a change in the measured signal. Controlling the probe density, probe distribution, reducing the non-specific interactions (noise) by controlling the surface structures will enable improvement of the functionalized surfaces with extension to development of new biosensor transduction motifs. Characterizing the electrochemical interface has been the focus of our research efforts for many years. We have developed a unique in-situ fluorescence microscopic method for analyzing these interfaces while controlling the electrochemical potential. This is accomplished by using a fluorophore labelled adsorbate. The fluorescence measured depends on the separation of the fluorophore from the electrode surface with fluorescence quenched when closer than 5nm. Our method enables a detailed understanding of the structure of these modified surfaces, from large scales (sub mm) to molecular sized regimes (10s of nm) and how this depends on the surface crystallography and the functionalization method (multiple step adsorption, co-adsorption, potential-controlled adsorption, chemical modification of the functionalized surface). We continue to advance our in-situ characterization method to include confocal microscopy with fluorescence lifetime imaging. These new analysis methods will be used in creating complex surfaces; from multi-component surfaces, to quantum dot decorated surfaces. Our long term goal is to devise methods for creating biosensor surfaces which are optimally functional guided by the use of advanced in-situ characterization techniques. This is the foundational principle which we will use to influence the creation of well defined biosensors and, more broadly all types of functionalized surfaces.

电化学工艺构成了许多新技术（例如电池，燃料电池，太阳能电池）以及生物分析传感的方法的基础。这些传感器的便携性和较小的占地面积 基于电化学的生物传感器依赖于用生物活性物种（例如脂质，核酸或酶）对电极表面的修饰，这些物种可以特异性地与样品中感兴趣的化合物特异性相互作用，然后将这种相互作用转换为可以准确测量的信号。这需要对传感器表面制备的高度控制。生物传感器必须在表面上具有足够数量的探针，这些探针可以由溶液中的目标访问，以便在专门交互时会在测量的信号中产生更改。通过控制表面结构来控制探针密度，探针分布，减少非特异性相互作用（噪声），将使功能化表面改善，并扩展到新的生物传感器转导基序的发展。 多年来，表征电化学界面一直是我们研究工作的重点。我们开发了一种独特的原位荧光显微镜方法，用于分析这些界面，同时控制电化学潜力。这是通过使用标有吸附物的荧光团来完成的。测得的荧光取决于荧光表面的分离，荧光在靠近5nm时猝灭。我们的方法可以详细了解这些修饰的表面的结构，从大尺度（亚MM）到分子大小的状态（NM的10s），以及这如何取决于表面晶体学和功能化方法（多步吸附，共吸附，吸附，电位控制，潜在的吸附，化学效果的化学改性）。我们继续推进我们的原位表征方法，包括荧光寿命成像的共聚焦显微镜。这些新的分析方法将用于创建复杂的表面。从多组分表面到量子点装饰的表面。我们的长期目标是设计用于创建生物传感器表面的方法，这些方法在使用先进的原位表征技术的最佳功能指导下。这是我们将用来影响定义良好的生物传感器以及更广泛的所有类型功能化表面的基本原理。