Strain engineering and bioprocessing strategies for bio-based production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) using cheap feedstocks

使用廉价原料生物生产聚（3-羟基丁酸酯-co-3-羟基戊酸酯）（PHBV）的菌株工程和生物加工策略

基本信息

批准号：
539590-2019
负责人：
Chou, CPerry
金额：
$ 10.96万
依托单位：
University of Waterloo
依托单位国家：
加拿大
项目类别：
Alliance Grants
财政年份：
2019
资助国家：
加拿大
起止时间：
2019-01-01 至 2020-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=693847
关键词：
Strain engineering bioprocessing strategies bio

项目摘要

Due to unstable supply of fossil fuels, numerous environmental concerns of climate change, stringent regulations for petro-based production, and motivation to "low carbon" economy, replacing petrochemical processes with biomass-biological strategies has been recognized as a modern technology for renewable, sustainable, and clean manufacturing of value-added chemicals and fuels. Polyhydroxyalkanoates (PHAs) are natural polyesters recently attracting considerable interests as promising candidates to replace petro-based synthetic plastics with numerous environmental impacts. Based on common interests in bio-based production of PHAs, our biomanufacturing research group at UWaterloo and Genecis Bioindustries, a Toronto-based company, initiate the proposed research with a major objective to develop an advanced whole-cell transformation platform, including genetically and metabolically engineered bacterial strains, and their associated bioprocessing strategies, for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), a biocopolymer member of PHAs, using cheap feedstocks, such as glycerol and volatile fatty acids (VFAs). Genecis has recently decided to explore PHBV-related technologies upon recognizing certain limitations associated with their existing PHA bioprocess and products. The developed engineered bacterial strains and bioprocesses for PHBV production can be readily transferred to Genecis for large-scale production, benefiting the existing Canadian biopolymer industry not only technologically but also economically. The proposed research is also highly multidisciplinary in biological sciences and industrial biotechnology with the following envisioned outcomes and benefits: (1) developing a clean, renewable, sustainable, and economically feasible PHBV bioprocess; (2) boosting Canada's research capacity and technological development in bioprocessing strategies and biopolymer production; (3) improving Canada's living environment and mitigating greenhouse gas emissions through clean biomanufacturing; and (4) training highly qualified personnel (HQP) for meeting challenges of the next generation of industrial biotechnology.

由于化石燃料的供应不稳定，气候变化的众多环境问题，基于石油的生产的严格法规以及“低碳”经济的动机，用生物量生物量策略代替石化过程，被认为是一种现代技术，是一种现代技术，可再生，可持续性，可持续性，并清理增添了吸收的化学品和燃料的制造。多羟基烷烃（PHAS）是自然的聚酯，最近吸引了相当大的兴趣，作为有前途的候选人，可以替代基于石油的合成塑料，并产生许多环境影响。基于基于生物的PHA的共同兴趣，我们在Uwaterloo的生物制造研究小组和一家位于多伦多的公司Genecis Bioindustries启动了拟议的研究，其主要目标是开发先进的全细胞转换平台，包括基因和代谢工程的细菌菌株及其相关的生物普罗普利亚策略，以及其相关的生物普罗普利亚策略PHAS的生物聚合物成员，使用廉价的原料，例如甘油和挥发性脂肪酸（VFAS），聚（3-羟基丁酸-CO-CO-CO-3-羟化反应）（PHBV）。 Genecis最近决定探索与PHBV相关的技术，因为他们认识到与现有的PHA生物处理和产品相关的某些局限性。可以轻松地将开发的用于PHBV生产的工程细菌菌株和生物处理能力转移到Genecis进行大规模生产，从而使现有的加拿大生物聚合物行业不仅在技术上而且在经济上也受益。拟议的研究在生物科学和工业生物技术学中也具有高度的多学科，并具有以下设想的结果和益处：（1）开发一种干净，可再生，可持续性，可持续性且经济上可行的PHBV生物普罗普斯；（2）增强加拿大在生物处理策略和生物聚合物生产中的研究能力和技术发展；（3）改善加拿大的生活环境并通过清洁生物制造来减轻温室气体的排放；（4）培训高素质的人员（HQP），以应对下一代工业生物技术的挑战。