Functional genomics of Pseudozyma flocculosa, a biocontrol agent of powdery mildews

白粉病生物防治剂絮状假酵母的功能基因组学

基本信息

批准号：
RGPIN-2016-05235
负责人：
Bélanger, Richard
金额：
$ 3.72万
依托单位：
Université Laval
依托单位国家：
加拿大
项目类别：
Discovery Grants Program - Individual
财政年份：
2019
资助国家：
加拿大
起止时间：
2019-01-01 至 2020-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=690248
关键词：
Functional genomics Pseudozyma flocculosa biocontrol

项目摘要

Practical application of biological control of plant pathogens presupposes a thorough understanding of the genetic, molecular and ecological events underlying such phenomena. This challenge has been the focal point of the research activities carried out in the applicant's laboratory over the last 25 years. In the previous installment, we have made some major strides toward elucidating the complex factors governing the biocontrol properties of Pseudozyma flocculosa, a natural antagonist of powdery mildew fungi. Of particular significance, we reported the following: 1) the discovery of genes and a gene cluster involved in flocculosin biosynthesis and establishment of an unsuspected close link with the phylogenetically related plant pathogen model fungus Ustilago maydis; 2) the association of specific enzymatic activities distinguishing the glycolipid structures produced by P. flocculosa and U. maydis; 3) the complete sequencing and genomic annotation of P. flocculosa, the first such report for a biocontrol agent; and 4) the comparative analysis of P. flocculosa genome with that of related Ustilaginales, U. maydis, Sporisorium reilianum and U. hordei, aimed at identifying the genetic factors that confer specific lifestyles in fungi. This latter achievement is perceived as a major breakthrough since it opens new opportunities to understand the subtle factors that make phylogenetically related fungi adopt opposite lifestyles. In the context of this application and in direct link with our recent progress, the objectives are: 1) to carry out the first full transcriptomic analysis of a tritrophic interaction BCA-fungal pathogen-plant using the system P. flocculosa-Blumeria graminis-barley; 2) with a specific emphasis on the unique factors discovered in the genome of P. flocculosa, to identify the determinant(s) that confer P. flocculosa, its biocontrol activity on powdery mildew fungi; 3) to identify the functional virulence factors of powdery mildew fungi by deductive analysis of their repression under P. flocculosa attack; 4) to understand the plant's role in sustaining the BCA-plant pathogen interaction; and 5) to conduct functional analyses of biocontrol determinants (genes) through reverse genetics in P. flocculosa and/or complementation in U. maydis. Fulfillment of these objectives will generate novel and distinctive information with regards to: the genetic determinants that regulate the biocontrol activity of P. flocculosa and those that regulate the pathogenicity of powdery mildew fungi against plants, and how expression (or repression) of these determinants influence the ecology of fungi. These results will have far reaching impact into the field of plant pathology as our model will provide unique data into the intrinsic genetic factors that separate and modulate two phylogenetically related fungi to behave as biocontrol agent or plant pathogen.**

植物病原体生物防治的实际应用预示着对这些现象背后的遗传、分子和生态事件的透彻理解。这一挑战一直是申请人实验室过去 25 年开展的研究活动的焦点。在上一部分中，我们在阐明控制絮状假酵母（白粉病真菌的天然拮抗剂）生物防治特性的复杂因素方面取得了一些重大进展。特别重要的是，我们报道了以下内容：1）发现了参与絮凝素生物合成的基因和基因簇，并与系统发育相关的植物病原体模型真菌玉米黑粉菌建立了意想不到的密切联系； 2) 区分由絮状假单胞菌和玉米淀粉产生的糖脂结构的特定酶活性的关联； 3) 絮状假单胞菌的完整测序和基因组注释，这是生物防治剂的第一份此类报告； 4）絮状 P. floculates 与相关黑粉菌目、玉米 U. maydis、Sporisorium reilianum 和 U. hordei 基因组的比较分析，旨在鉴定赋予真菌特定生活方式的遗传因素。后一项成就被认为是一项重大突破，因为它为理解使系统发育相关的真菌采取相反生活方式的微妙因素提供了新的机会。在本申请的背景下，并与我们最近的进展直接相关，目标是：1) 使用 P. flocylosa-Blumeria graminis-大麦系统对 BCA-真菌病原体-植物三营养相互作用进行首次完整转录组分析; 2) 特别强调在絮状白粉病基因组中发现的独特因素，以确定赋予絮状白粉病其对白粉病真菌生物防治活性的决定因素； 3）通过演绎分析白粉病真菌在絮状白粉病攻击下的抑制作用，鉴定白粉病真菌的功能毒力因子； 4) 了解植物在维持BCA-植物病原体相互作用中的作用； 5) 通过絮状疫病菌的反向遗传学和/或玉米须菌的互补对生物防治决定因素（基因）进行功能分析。这些目标的实现将产生以下方面的新颖且独特的信息：调节絮状白粉病生物防治活性的遗传决定因素和调节白粉病真菌对植物的致病性的遗传决定因素，以及这些决定因素的表达（或抑制）如何影响真菌的生态学。这些结果将对植物病理学领域产生深远的影响，因为我们的模型将为内在遗传因素提供独特的数据，这些因素可分离和调节两种系统发育相关的真菌，使其充当生物防治剂或植物病原体。**