Investigation of different column packing methods on the efficiency of chromatographic separation for biotechnology and pharmaceutical applications

研究不同柱填充方法对生物技术和制药应用色谱分离效率的影响

• 批准号: 530871-2018
• 负责人: Bassi, Amarjeet
• 金额: $ 2.91万
• 依托单位: University of Western Ontario
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=687827
• 关键词: Investigation; different; column; packing; methods

Preparative liquid chromatography is the dominant, downstream operation for the purification ofbiological compounds in the bio-pharmaceutical industries. The packed bed column is essential to the performance of the chromatographic step. As new column packing methods are developed, it is necessary to qualify columns to ensure they perform in a robust and consistent fashion. In addition, as new analytical procedures and preparative scale approaches are developed, improved methods are needed for column packing or modifications to current approaches are required. Over the past few years, the industrial partner in this application, PolyAnalytik Inc., has been a part of several research and development projects (R&D) in the pharmaceutical and medical device industries. PolyAnalytik Inc. has also been involved in selling chromatographic columns in the separation of small molecules and macromolecules for a decade. The extensive knowledge and experience gained by the organization in the purification and chromatographic techniques used in pharmaceuticals applications have allowed the organization to consider the development of uniquely packed chromatographic columns containing bioprocessing resins for many purification applications (e.g. for pharmaceuticals such as proteins, peptides, antiviruses). PolyAnalytik Inc. owns several proprietary approaches for column packing. In this proposal, the Bassi lab will explore experimental and mathematical modelling tools to investigate the packed column efficiency of these columns in collaboration with Polyanalytik to further advance the development of highly efficient systems. This investigative study on column packing efficiencies in partnership with the Bassi laboratory at UWO is crucial to Polyanalytik as the development of such packed columns as this will lead to innovative and distinctively packed columns for high efficiency chromatographic applications.

制备液相色谱法是生物制药产业中生物学化合物纯化的主要下游操作。包装的床柱对于色谱步骤的性能至关重要。随着新的列包装方法的开发，有必要限定列以确保它们以强大且一致的方式执行。此外，随着新的分析程序和制备量表方法的开发，需要改进的方法来对当前方法进行列或修改。在过去的几年中，该应用程序的工业合作伙伴Polyanalytik Inc.一直是制药和医疗设备行业的几个研发项目（R＆D）的一部分。 十年来，Polyanalytik Inc.也参与了在小分子和大分子分离中销售色谱柱。 该组织在药品应用中使用的纯化和色谱技术获得的广泛知识和经验使组织可以考虑开发许多含有许多纯化应用的含有生物处理树脂的独特包装的色谱柱（例如，用于诸如蛋白质，肽，肽，抗病毒等药物的药物）。 Polyanalytik Inc.拥有多种专有方法。在该提案中，巴西实验室将探索实验和数学建模工具，以与Polyanalytik合作研究这些列的填充柱效率，以进一步推动高效系统的开发。这项针对与UWO的Bassi实验室合作的柱填料效率的研究研究对聚隔离学至关重要，因为这将导致高效色谱应用的创新和独特的柱子的开发，这将导致创新且独特的柱子。