Design of polymers and bioreactors in the development of a tissue engineering strategy for degenerative disc disease

退行性椎间盘疾病组织工程策略开发中的聚合物和生物反应器设计

• 批准号: RGPIN-2015-05179
• 负责人: Amsden, Brian
• 金额: $ 2.55万
• 依托单位: Queen's University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=685474
• 关键词: Design; polymers; bioreactors; development; tissue

The major challenge addressed in the proposed program is the design, synthesis, and testing of new polymeric biomaterials and bioreactors to be used for the development of a new treatment for degenerative intervertebral disc disease. The intervertebral disc is the connective tissue between the vertebral bones of the spine. It holds the verterbrae together, absorbs compressive loads, and allows the spine to rotate, bend, and twist. Degeneration of the intervertebral disc is a common source of chronic pain and reduced quality of life in people over the age of 40. About 12 million patients are diagnosed with this condition annually in North America. Treatment is typically palliative; however, in ~ 10% of these patients, surgery is ultimately necessary. Current surgical methods provide temporary relief, and do not repair the damaged disc. A treatment methodology is proposed that involves removing the degenerating tissue, and replacing it with regenerative cells delivered within a polymer biomaterial that can form a cell supportive depot while providing mechanical support to the damaged disc. The regenerative cells will ultimately produce replacement tissue while at the same time the polymer biomaterial degrades. The regenerative cells will be obtained from stem cells isolated from fat. These stem cells will be induced to become cells typical of the centre of the intervertebral disc (nucleus pulposus cells) in a bioreactor for this purpose designed based on the cell and tissue biology of the disc. The polymer biomaterials used to deliver the cells will be designed to support and substantiate the transformed stem cells into nucleus pulposus cells while ultimately degrading into products that are biocompatible and readily eliminated from the body.**

拟议项目中解决的主要挑战是设计、合成和测试新型聚合物生物材料和生物反应器，用于开发退行性椎间盘疾病的新疗法。椎间盘是椎骨之间的结缔组织。它将椎骨固定在一起，吸收压力负荷，并允许脊柱旋转、弯曲和扭曲。椎间盘退变是慢性疼痛和质量下降的常见原因。 40 岁以上人群的生活受到影响。在北美，每年约有 1200 万患者被诊断出患有这种疾病，但其中约 10% 的患者最终需要手术治疗才能获得暂时缓解。提出了一种治疗方法，涉及去除退化组织，并用聚合物生物材料内的再生细胞替代它，该聚合物生物材料可以形成细胞支持库，同时为受损的椎间盘提供机械支撑。最终将产生替代品再生细胞将从脂肪中分离出来，并在生物反应器中被诱导成为椎间盘中心的典型细胞（髓核细胞）。这一目的是基于椎间盘的细胞和组织生物学而设计的，用于输送细胞的聚合物生物材料将被设计为支持和证实转化的干细胞为髓核细胞，同时最终降解为生物相容性产品。并很容易从体内排出。**