New microscale sample processing approaches for application to proteomics and bioanalysis

适用于蛋白质组学和生物分析的新型微量样品处理方法

• 批准号: RGPIN-2016-06636
• 负责人: Waldron, Karen
• 金额: $ 1.82万
• 依托单位: Université de Montréal
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=667971
• 关键词: New; microscale; sample; processing; approaches

The health and the well-being of Canadians rely not only on accurate diagnosis and effective treatment but also a pollution-free environment in which to live. Chemical analysis plays a key role in such goals by helping uncover the causes of disease, or by following a patient's drug therapy response, or by testing water, soil and air for toxins to both plants and animals. By far the most common methods for said chemical analyses are grouped under the term “analytical separations”, encompassing the powerful techniques of chromatography, electrophoresis and mass spectrometry. The complexity of samples submitted for chemical analysis, sometimes containing over a thousand different compounds, is such that rigorous sample preparation techniques are required prior to the analytical separation. In fact, the selectivity and detection sensitivity of the above-mentioned analytical separation methods improve greatly with preparation techniques tailored to the analysis. Therefore, our research program is dedicated to developing and optimizing sample preparation methods with specific application to microliter scale bioanalysis and towards enhancing the power of state-of-the-art analytical separations. ***The identification of proteins and the understanding of their biological interactions is key to developing diagnostic and therapeutic strategies. Therefore, we are designing sample preparation methods that aid in protein determination, including proteins at low abundance because these are the ones suspected to have relevance for disease diagnosis. We are developing fractionation strategies and enzymatic methods that render proteins easier to analyze by mass spectrometry and address the current trend towards miniaturization. Capillary electrophoresis is our main microscale method, which is ideally suited because nanolitre volumes can be injected, it is easily automated and it can be translated into microchip format for additional miniaturization. Our immobilized enzyme microreactor methods are being developed to allow simple construction and rapid protein digestion for better mass spectrometry analysis. Innovative combinations of these microscale techniques and methods applied to minute sample volumes has the long term potential to impact disease diagnosis and monitoring in humans and animals. **

加拿大人的健康和福祉不仅依赖于准确的诊断和有效的治疗，而且还依赖于无污染的生活环境，化学分析通过帮助揭示疾病的原因或遵循以下原则在实现这些目标中发挥着关键作用。患者的药物治疗反应，或通过测试水、土壤和空气中植物和动物的毒素，到目前为止，最常见的化学分析方法被归为“分析分离”这一术语，包括色谱、电泳等强大技术。和质量提交化学分析的样品有时含有一千多种不同的化合物，因此在分析分离之前需要严格的样品制备技术。事实上，上述分析分离方法的选择性和检测灵敏度。因此，我们的研究计划致力于开发和优化特定于微升规模生物分析的样品制备方法，并增强最先进的分析分离能力***。蛋白质的鉴定及其理解因此，我们正在设计有助于蛋白质测定的样品制备方法，包括低丰度蛋白质，因为这些蛋白质被怀疑与疾病诊断相关。使蛋白质更容易通过质谱分析并解决当前小型化趋势的方法是我们的​​主要微尺度方法，该方法非常适合，因为可以注射纳升体积，易于自动化，并且可以转化为微芯片格式。我们正在开发固定化酶微反应器方法，以实现简单的结构和快速的蛋白质消化，从而实现更好的质谱分析，这些微尺度技术和方法的创新组合应用于微小样品量具有影响疾病诊断和监测的长期潜力。在人类和动物中。