Temperature-sensitive proteases for research and diagnostics

用于研究和诊断的温度敏感蛋白酶

• 批准号: 523350-2018
• 负责人: Nano, Francis
• 金额: $ 9.07万
• 依托单位: University of Victoria
• 依托单位国家: 加拿大
• 项目类别: Idea to Innovation
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=660631
• 关键词: Temperature; sensitive; proteases; research; diagnostics

The objective of this proposal is to develop a family of temperature-sensitive proteases that can be used in molecular biology research and in automated diagnostic systems to detect human diseases. A protease is an enzyme that attacks and degrades other proteins. Often in the research or diagnostic laboratory a series of steps are used, and each step uses a different enzyme, which is a protein. In these situations a major challenge is to remove the enzyme from one step before moving on to the next step. A temperature-sensitive protease addresses this problem by providing a way of removing an enzyme through degradation, and then removing the protease activity through mild heating. Currently, there are no temperature-sensitive proteases commercially available, thus our discovery will fill a currently unmet need.

该提案的目的是开发一个对温度敏感蛋白酶的家族，可用于分子生物学研究和自动诊断系统中以检测人类疾病。 蛋白酶是攻击其他蛋白质的酶。 通常在研究或诊断实验室中使用一系列步骤，每个步骤都使用不同的酶，即一种蛋白质。 在这些情况下，一个主要的挑战是从一步中删除酶，然后再进入下一步。 温度敏感的蛋白酶通过提供通过降解去除酶的方法来解决此问题，然后通过轻度加热去除蛋白酶活性。 当前，尚无对温度敏感的蛋白酶的市售，因此我们的发现将满足当前未满足的需求。