Identification of Molecular Mechanisms underlying Reciprocal Responses in the Interaction between Arabidopsis and the Cell-Content-Feeding Chelicerate Herbivore Spider Mite

拟南芥与以细胞内容物为食的螯肢食草动物蜘蛛相互作用中相互反应的分子机制的鉴定

• 批准号: RGPIN-2018-04538
• 负责人: Grbic, Vojislava
• 金额: $ 3.42万
• 依托单位: University of Western Ontario
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=652380
• 关键词: Identification; Molecular; Mechanisms; underlying; Reciprocal

Plant-herbivore interactions evolved over millions of years resulting in an elaborate arms race between interacting species. While specialist herbivores evolved specific strategies to cope with the defenses of a limited number of hosts, our understanding of how generalist herbivores deal with the defenses of a plethora of diverse host plants is largely unknown. I have established the plant-herbivore interaction experimental system comprising of Arabidopsis (as a host plant) and an extreme generalist herbivore, the two spotted spider mite (Tetranychus urticae (Koch), TSSM). TSSM feeds on over 1100 plant species including more than 150 crops worldwide. With increased reproductive potential under conditions of global warming, combined with an outstanding ability to adapt to new crops and develop resistance to pesticides, the TSSM is becoming a high-risk pest threatening global crop security. In the last 5 years, my group: a) demonstrated TSSM's unique feeding pattern among other cell-content feeding arthropods, b) developed genomics and reverse genetics resources for TSSM (which combined with the resources already existing for the model plant Arabidopsis provide a unique opportunity to undertake simultaneous functional genomics analysis of both interacting organisms), and c) performed initial genome-wide reciprocal analysis of Arabidopsis and TSSM responses. In the next 5 years, we will build on previous knowledge and developed resources to address following research questions:***a) How Arabidopsis plants establish defenses in systemic unwounded tissues in the anticipation of TSSM feeding?***b) What is the function of genes that associate with Arabidopsis resistance to TSSM?***c) What is the mode of action of Arabidopsis defense compounds against TSSM?***The proposed multidisciplinary approach will combine plant genetics, and plant and mite physiology, molecular biology, transcriptomics and metabolomics. It is expected to uncover new regulators of plant defenses and plant compounds with acaricidal activity. In parallel, it will identify TSSM counter-adaptations to Arabidopsis defenses. This research will establish an unprecedented level of understanding of plant-herbivore interaction. Besides contribution to the fundamental knowledge, the knowledge of plant defense mechanisms can lead to development of new control strategies against TSSM that is an important agricultural pest, while mechanisms of mite counter adaptations will help maintain the efficacy of these control strategies in agricultural practices. Additionally, the proposed research will enable an outstanding training of HQPs, creating experts with cutting-edge skills in the highly valued area of agrigenomics and crop protection, in addition to transferable skills in commercialization, IP and communication.**

植物 - 动物的相互作用在数百万年内演变，导致相互作用物种之间的精心武器种族。尽管食草动物的专家发展了特定的策略，以应对有限数量的宿主的防御能力，但我们对通才食草动物如何处理多种寄主植物的防御能力的理解在很大程度上是未知的。我已经建立了包括拟南芥（作为宿主植物）和极端通才的草食动物的植物 - 动物相互作用实验系统，两个斑点的蜘蛛螨（Tetranychus tureticae（Koch），TSSM）。 TSSM以1100多种植物物种为食，包括全球150多种作物。随着在全球变暖条件下的生殖潜力增加，加上适应新作物并发展对农药的抗药性的出色能力，TSSM正成为一种高风险的害虫威胁全球作物的安全。 In the last 5 years, my group: a) demonstrated TSSM's unique feeding pattern among other cell-content feeding arthropods, b) developed genomics and reverse genetics resources for TSSM (which combined with the resources already existing for the model plant Arabidopsis provide a unique opportunity to undertake simultaneous functional genomics analysis of both interacting organisms), and c) performed initial genome-wide reciprocal analysis of Arabidopsis and TSSM响应。在接下来的5年中，我们将基于以前的知识和开发资源来解决以下研究问题：*** a）拟南芥植物如何在预期TSSM喂食的情况下在系统性未造成的组织中建立防御能力？结合植物遗传学，植物和螨虫生理学，分子生物学，转录组学和代谢组学。预计将发现具有杀菌活性的植物防御和植物化合物的新调节剂。同时，它将确定TSSM对拟南芥防御能力的反调整。这项研究将建立对植物 - 赫生相互作用的前所未有的理解水平。除了对基本知识做出贡献外，植物防御机制的知识还可以导致针对TSSM的新控制策略的发展，而TSSM是重要的农业害虫，而螨虫反适应的机制将有助于维持这些控制策略在农业实践中的功效。此外，拟议的研究还将对HQP进行出色的培训，除了在商业化，IP和沟通方面可转移技巧外，还可以在高度价值的农业类和作物保护领域中创建具有尖端技能的专家。**