Characterization of Dbf4/Cdc7 function in DNA replication and cell cycle checkpoint responses

DNA 复制和细胞周期检查点反应中 Dbf4/Cdc7 功能的表征

• 批准号: RGPIN-2015-06265
• 负责人: Duncker, Bernard
• 金额: $ 2.77万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=649770
• 关键词: Characterization; Dbf4; Cdc7; function; DNA

My research group is using budding yeast in studies of cell division. We are currently focusing our investigations on identifying and characterizing protein factors that control the initiation of DNA replication (duplication of the genetic material). Budding yeast has proven to be a very useful organism for such studies because it is one of the few species for which the starting points for DNA replication (origins of replication) have been well characterized. This has facilitated the identification of numerous protein factors that regulate these replication origins. Dbf4 and Cdc7 are two such proteins which work together to trigger replication. In addition to their function in DNA replication, Dbf4 and Cdc7 are important for cellular 'checkpoint' functions, which prevent duplication of DNA when it has been damaged. Research in my lab is centered on a better understanding of the way these proteins work together, and interact with other proteins in the cell, to carry out their essential roles in cell division. Importantly, versions of Dbf4 and Cdc7 appear to exist and function in much the same way in cells from a wide variety of species. Therefore, the  results of these studies will help us better determine how organisms, including humans, grow and develop, as well us help us understand how cells respond when they are exposed to environmental pollutants that cause DNA damage. **

我的研究小组正在研究细胞分裂研究中。目前，我们将投资集中在识别和表征控制DNA复制启动（遗传材料复制）的蛋白质因子上。事实证明，萌芽的酵母是此类研究非常有用的组织，因为它是为数不多的DNA复制起点（复制的起源）的物种之一。这已经准备好鉴定了调节这些复制起源的众多蛋白质因子。 DBF4和CDC7是两个这样的蛋白质，它们共同触发复制。除了它们在DNA复制中的功能外，DBF4和CDC7对于细胞“检查点”函数也很重要，该功能可防止DNA在受损时重复。在我的实验室中的研究以更好地理解这些蛋白质的工作方式，并与细胞中的其他蛋白质相互作用，以在细胞分裂中发挥基本作用。重要的是，DBF4和CDC7的版本似乎存在，并且在各种物种的细胞中以几乎相同的方式起作用。因此，这些研究的结果将有助于我们更好地确定包括人类，生长和发育在内的生物体如何帮助我们了解细胞对造成DNA损伤的环境污染物时的反应。 **