Detection of EPS

EPS检测

• 批准号: 530812-2018
• 负责人: Blight, Barry
• 金额: $ 1.82万
• 依托单位: University of New Brunswick
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=645632
• 关键词: Detection; EPS

This work will result in the development of a new single molecule fluorescence sensor or extracellular**polymeric substance (EPS) which is a biofilm forming residue that can contain microbial cultures. This work**will have widespread interest in domestic water supplies, industrial wastewater, industrial fluid transfer**processes, and in wastewater treatment. In collaboration with LuminUltra Technologies Ltd, experimental**development and evaluation will be conducted in the research lab of Prof Blight at the University of New**Brunswick and conducted by one graduate student and one research assistant. We will 1) develop a single**molecule fluorescent sensor for EPS; 2) we will test at UNB on comparable materials such as dextrose and**amylopectin; 3) we will then test EPS in the research quarters, on-site at LuminUltra Technologies. There will**be a five-fold benefit to Canada when this collaboration proves successful: 1) Public Safety - benefit of early**detection of biofilm scaffolds that support harmful microbial deposition of domestic water infrastructure; 2)**Infrastructure retention - prevention of MIC and the 'dolomite problem' will extend the lifespan (cost savings)**of domestic water infrastructure; 3) Industry - many industries require microbes for some production process**and/or must prevent microbe growth for others. Our technology will allow rapid detection for biofilm**deposition prevention (safety and cost savings); 4) Science Impact - a product of this type, developed in**Canada, will make a significant impact on profile of Canadian Science; 5) Economy - the global addressable**market for this product is $1.3 billion worldwide, with potential use in any country, and being both developed**and produced in Canada furthers this impact.

这项工作将导致开发一种新的单分子荧光传感器或细胞外**聚合物物质（EPS），它是一种可以包含微生物培养物的生物膜形成残留物。这项工作**将在生活供水、工业废水、工业流体输送**过程和废水处理方面引起广泛的兴趣。与 LuminUltra Technologies Ltd 合作，实验**开发和评估将在新**不伦瑞克大学 Blight 教授的研究实验室中进行，由一名研究生和一名研究助理进行。我们将1）开发用于EPS的单**分子荧光传感器； 2) 我们将在 UNB 对类似材料进行测试，例如葡萄糖和**支链淀粉； 3) 然后我们将在 LuminUltra Technologies 的研究区现场测试 EPS。当这次合作成功时，加拿大将获得五倍的好处： 1) 公共安全 - 早期**检测支持家庭供水基础设施有害微生物沉积的生物膜支架的好处； 2)**基础设施保留 - 预防 MIC 和“白云石问题”将延长国内供水基础设施的使用寿命（节省成本）**； 3) 工业 - 许多行业的某些生产过程需要微生物**和/或必须防止其他行业的微生物生长。我们的技术将允许快速检测生物膜**沉积预防（安全和节省成本）； 4) 科学影响——此类产品在**加拿大开发，将对加拿大科学界产生重大影响； 5) 经济 - 该产品的全球潜在**市场为 13 亿美元，在任何国家都有潜在用途，并且在加拿大开发**和生产进一步扩大了这种影响。