Studies of the effects of phosphorylation and protein interactions on ATP-binding casette transporter activity

磷酸化和蛋白质相互作用对 ATP 结合盒转运蛋白活性影响的研究

• 批准号: RGPIN-2015-05372
• 负责人: Kanelis, Voula
• 金额: $ 2.19万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=630014
• 关键词: Studies; effects; phosphorylation; protein; interactions

The protein Ycf1p is a member of a large superfamily of membrane proteins found in all organisms. By utilizing the energy of ATP, known as the energy currency of the cell, these proteins actively transport solutes into and out of the cell, regulate the function of other proteins, or open pores or channels to allow charged species to pass through. These ATP-using membrane proteins have a conserved molecular architecture that contains two regions embedded in the cellular envelope (known as the membrane) and two regions located inside the cell (known as the cytoplasm). Proper regulation of this process is essential, as many genetic diseases, including cystic fibrosis and adrenoleukodystrophy, result from dysfunction of different proteins in this ubiquitous superfamily.

Ycf1p 蛋白是所有生物体中发现的膜蛋白大超家族的成员。通过利用被称为细胞能量货币的 ATP 能量，这些蛋白质主动将溶质转运进出细胞，调节其他蛋白质的功能，或打开孔或通道以允许带电物质通过。这些使用 ATP 的膜蛋白具有保守的分子结构，其中包含嵌入细胞包膜的两个区域（称为膜）和位于细胞内部的两个区域（称为细胞质）。对该过程的适当调节至关重要，因为许多遗传疾病，包括囊性纤维化和肾上腺脑白质营养不良，都是由这个普遍存在的超家族中不同蛋白质的功能障碍引起的。