Development of a novel automated micropropagation system for plant tissue culture

开发用于植物组织培养的新型自动化微繁殖系统

• 批准号: 474550-2014
• 负责人: Park, Edward
• 金额: $ 3.64万
• 依托单位: Simon Fraser University
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=618768
• 关键词: Development; novel; automated; micropropagation; system

Micropropagation is an advanced vegetative propagation technique for producing a large number of genetically uniform and pathogen-free transplants in a limited time and space. It is a plant tissue culture technique for growing plants from small pieces of tissue under sterile conditions in a laboratory on specially selected media. The benefits of plant tissue culture are extensive in the agricultural world in many respects, the first being that it allows for the production of huge numbers of plants in a very short period of time. Plant tissue culture is also advantageous to growers because the overwhelming number of disease free plants can be produced using the tissue collected from a single parent plant - a plant which itself remains unharmed in the tissue harvesting process. Propagation through tissue culture also eliminates the possibility of any interruption in the growing season because it can be carried out inside the carefully regulated controlled environment. However, plant tissue culture or micropropagation is a capital-intensive industry, and in some cases the unit cost per plant becomes unaffordable. Due to the increased cost of labour in the developed countries, the conventional micropropagation system has been challenged by high production cost and low gross profit. In general, many micropropagation companies in high cost labor areas have had to either automate their system to reduce labour requirements or move operations to low labour cost area to reduce their production cost. The main objective of this project is to development an automated micropropagation system capable of handling plant tissue cultures. Robotic technologies developed in the automotive industry are starting to drive innovation in horticulture for the goal of improving productivity and reducing labour costs. The main challenge of automating the micropropagation process lies in the required fine manipulation of fragile micro-shoots (e.g., of blueberry stocks). However, the capability of automation systems has dramatically increased over the last decade with new sensing and actuation technologies, allowing us to address this challenge and propose an automated micropropagation solution that can boost efficiencies and reduce labour costs of tissue culturing.

微爆是一种先进的营养传播技术，用于在有限的时间和空间中产生大量遗传统一和无病原体移植。这是一种在特殊选择培养基的实验室中，在无菌条件下，在无菌条件下从小块组织中种植植物的一种植物组织培养技术。在许多方面，在农业世界中，植物组织培养的好处是广泛的，第一个是它允许在很短的时间内生产大量植物。植物组织培养对于种植者也是有利的，因为可以使用从单亲植物中收集的组织生产大量的无疾病植物 - 这种植物本身在组织收集过程中仍然没有受到伤害。通过组织培养的传播还消除了生长季节中任何中断的可能性，因为它可以在经过精心调节的受控环境中进行。但是，植物组织培养或微繁殖是一个资本密集型行业，在某些情况下，每工厂的单位成本变得无法承受。由于发达国家的劳动成本增加，传统的微繁殖系统受到高生产成本和毛利率低的挑战。通常，许多高成本劳动力地区的微型繁殖公司不得不自动化其系统以减少劳动力需求，或者将运营转移到低人工成本领域，以降低其生产成本。该项目的主要目的是开发能够处理植物组织培养物的自动化微繁殖系统。汽车行业开发的机器人技术开始推动园艺创新，以提高生产率和降低人工成本。自动化微繁殖过程的主要挑战在于需要精细操纵脆弱的微型射击（例如，蓝莓库存）。但是，在过去的十年中，通过新的感应和驱动技术，自动化系统的能力大大提高，使我们能够应对这一挑战并提出一种自动化的微繁殖解决方案，该解决方案可以提高效率并降低组织培养的劳动力成本。