Microbial community response to biostimulation in a petroleum hydrocarbon contaminated site in Saskatoon, SK

萨斯卡通石油碳氢化合物污染场地微生物群落对生物刺激的反应

• 批准号: 491896-2015
• 负责人: McBeth, Joyce
• 金额: $ 1.82万
• 依托单位: University of Saskatchewan
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=587129
• 关键词: Microbial; community; response; biostimulation; petroleum

We propose to characterize and develop a conceptual model for microbial community changes associated with biostimulation of a hydrocarbon contaminated aquifer in Saskatoon, SK. The aquifer was contaminated by a fuel station over a period of several decades, and is one of many similar sites managed by the industry partner on this project, Federated Cooperatives Limited (FCL). These sites present a long-term liability for FCL's member co-operatives, and they are keen to refine the approaches they use to remediate these sites. To achieve this objective, it is important to have a holistic understanding of the interplay between the local geology, the distribution of the contaminants, the microbiology of the aquifer groundwater and soils, and the impact of biostimulation amendments on the system through time. We will use high-throughput amplicon sequencing to characterize microbial community changes in the groundwater at the site over a 6 month biostimulation period. We will also characterize core samples taken from the site to examine the in-situ microbiology and mineralogy in the soils prior to biostimulation.These data, along with geochemical analyses provided by FCL, will be used to construct a conceptual model for the biogeochemical changes driven by biostimulation in the aquifer. This will help us to build our understanding of how microbes respond to biostimulation at this site and and improve the strategies FCL uses for remediation of this and similar sites moving forward.

我们建议描述和开发一个概念模型，用于描述与萨斯喀彻温省萨斯卡通碳氢化合物污染含水层的生物刺激相关的微生物群落变化。该含水层几十年来一直受到加油站的污染，是该项目的行业合作伙伴 Federated Cooperatives Limited (FCL) 管理的许多类似地点之一。这些场地给 FCL 成员合作社带来了长期责任，他们热衷于改进修复这些场地的方法。为了实现这一目标，重要的是全面了解当地地质、污染物分布、含水层地下水和土壤的微生物学以及生物刺激修正随时间对系统的影响之间的相互作用。我们将使用高通量扩增子测序来表征 6 个月生物刺激期内该地点地下水中微生物群落的变化。我们还将对从现场采集的岩心样本进行表征，以在生物刺激之前检查土壤中的原位微生物学和矿物学。这些数据以及 FCL 提供的地球化学分析将用于构建驱动生物地球化学变化的概念模型通过含水层中的生物刺激。这将帮助我们了解微生物如何响应该地点的生物刺激，并改进 FCL 今后用于修复该地点和类似地点的策略。