Growth in the host: catabolic capacities of opportunistic bacteria and possible links to virulence

宿主中的生长：机会性细菌的分解代谢能力及其与毒力的可能联系

基本信息

批准号：
327054-2011
负责人：
Cardona, Silvia
金额：
$ 2.62万
依托单位：
University of Manitoba
依托单位国家：
加拿大
项目类别：
Discovery Grants Program - Individual
财政年份：
2015
资助国家：
加拿大
起止时间：
2015-01-01 至 2016-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=571905
关键词：
Growth host catabolic capacities opportunistic

项目摘要

Bacteria are ubiquitous in nature, present in great diversity and quantity in all soils and water. Many environmental bacteria are harmless to animals and man, but others can jump from the environment to the human host and become extremely pathogenic. Infections with the soil bacteria Burkholderia cepacia complex have become a serious risk for people with the genetic disorder cystic fibrosis. We propose that that these opportunistic bacteria utilize nutrients to produce signal molecules that have a pathogenic effect to the host. In this research program, we will investigate which compounds present in the soil and in the human host serve as nutrients and at the same time elicit a pathogenic response in Burkholderia cepacia complex. We will create a large collection of bacterial mutants, defective in the use of certain nutrients and investigate if these mutants are less pathogenic. Thanks to B. cepacia complex's ability to colonize diverse hosts, we will be able to use host models like the plant Arabidopsis and the worm C. elegans, which allow screening hundreds of mutants in a short period of time. Our research will contribute to our general understanding of the host as a growth environment where the host nutrients could be exploited to build molecules that trigger pathogenic responses in opportunistic bacteria.

细菌本质上是普遍存在的，在所有土壤和水中都具有巨大的多样性和数量。许多环境细菌对动物和人无害，但其他环境细菌可以从环境跳到人类宿主并变得极为致病。土壤细菌的感染Burkholderia cepacia络合物已成为患有遗传疾病囊性纤维化患者的严重风险。我们建议这些机会细菌利用营养产生对宿主具有致病作用的信号分子。在该研究计划中，我们将研究在土壤和人类宿主中存在哪些化合物作为营养，同时引起了Burkholderia cepacia Complex中的致病反应。我们将创建大量的细菌突变体，在使用某些营养物质方面有缺陷，并研究这些突变体是否较少致病。得益于Cepacia B. ceply综合体能够定居多样的宿主，我们将能够使用诸如植物拟南芥和蠕虫秀丽隐杆线虫等宿主模型，这些模型允许在短时间内筛选数百个突变体。我们的研究将有助于我们对宿主作为一个生长环境的一般理解，在这种环境中，可以利用宿主营养物质来构建触发机会细菌中致病反应的分子。