Development of biocompatible coatings for magnetic nanoparticles

磁性纳米颗粒生物相容性涂层的开发

• 批准号: 312284-2010
• 负责人: Hafeli, Urs
• 金额: $ 1.68万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=571720
• 关键词: Development; biocompatible; coatings; magnetic; nanoparticles

Currently, the only approved application of magnetic particles is as contrast agents in magnetic resonance imaging (MRI). These particles, however, have great untapped potential. They, for example, could be used in many therapeutic applications including magnetically targeted anticancer therapy or magnetically controlled drug delivery to areas where it is difficult to deliver highly potent drugs (such as the eye) without having serious negative side effects on non-targeted organs. To enable therapeutic magnetic targeting, we plan to prepare magnetite nanoparticles with purposefully designed coatings that give them high stability, good biocompatibility, and a "hook" to attach to many different molecules such as antibodies, peptides, fluorescent dyes and drugs. These magnetic nanoparticles will be evaluated on their own and additionally encapsulated in larger magnetic microspheres. The uniform size of these microspheres makes them react in a predictable way to an external magnetic field while they flow through a patient's blood stream. Such predictability has been missing from currently available non-toxic microspheres as they are mixtures of many different sizes. To confirm the success of our strategy, magnetic nanoparticles and microspheres will be evaluated in cell culture and microscopy experiments, followed by an in vivo eye model of magnetic targeting.

目前，唯一批准的磁颗粒应用是磁共振成像（MRI）中的对比剂。但是，这些颗粒具有巨大的未开发潜力。例如，它们可用于许多治疗应用中，包括磁性靶向抗癌治疗或磁通的药物输送到难以输送高度有效的药物（例如眼睛）而不会对非定位器官产生严重负面影响的区域。为了实现治疗性磁靶向，我们计划制备具有有目的设计涂层的磁铁矿纳米颗粒，使它们具有很高的稳定性，良好的生物相容性和一个“钩子”，以附着在许多不同的分子上，例如抗体，肽，荧光染料和药物。这些磁性纳米颗粒将自行评估，并将其另外封装在较大的磁微球中。这些微球的均匀尺寸使它们以可预测的方式对外部磁场进行反应，同时它们流过患者的血液。当前可用的无毒微球是许多不同尺寸的混合物，因此缺少这种可预测性。为了确认我们的策略的成功，将在细胞培养和显微镜实验中评估磁性纳米颗粒和微球，然后在体内磁靶向模型。