Using TRFLP sequencing to assess and monitor beneficial and detrimental soil nematodes in agricultural systems

使用 TRFLP 测序评估和监测农业系统中有益和有害的土壤线虫

• 批准号: 474772-2014
• 负责人: Lindo, Zoë
• 金额: $ 1.66万
• 依托单位: University of Western Ontario
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=565086
• 关键词: Using; TRFLP; sequencing; assess; monitor

Understanding changes in free-living soil nematode assemblages under no-till and conventional tillage systems will allow better understanding of the relationship between soil biodiversity and changes in crop productivity. There is an overall need to develop biological indicators of soil health, and diagnose soil health status by determining ratios of beneficial and detrimental soil nematodes under different tillage and cropping systems. The development of a rapid and replicable molecular approach using TRFLP will improve the ability of growers to routinely monitor the soil nematode community. Yet TRFLP methods are current commercially unavailable to profile soil nematode communities. The realisation of these goals will allow A&L Laboratories (A&L) to make these tools available to growers so they can assess whether their agronomic practices have made their soil more productive via the development of beneficial soil nematodes. This premise is dependent

了解在无耕作和常规耕作系统下自由生活的土壤线虫组合的变化将可以更好地理解土壤生物多样性与作物生产率变化之间的关系。总体上需要开发土壤健康的生物学指标，并通过确定不同耕作和种植系统下有益和有害土壤线虫的比率来诊断土壤健康状况。使用TRFLP快速且可复制的分子方法的发展将提高种植者常规监测土壤线虫社区的能力。然而，TRFLP方法在剖面土壤线虫社区的商业上目前是不可用的。实现这些目标将使A＆L实验室（A＆L）能够使这些工具可用于种植者，因此他们可以评估他们的农艺实践是否通过开发有益的土壤线虫来使土壤更加生产力。这个前提取决于